Overview
- External ontology
- mediadive.medium:255 (source link unavailable) — CLOSTRIDIUM (GS) MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'gs_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:255', 'original_category': 'bacterial'}
- {'name': 'medium_255_modified_for_dsm_14427', 'source': 'KOMODO', 'source_id': 'komodo.medium:255_14427', 'original_category': 'bacterial'}
- {'name': 'medium_255_modified_for_dsm_8532', 'source': 'KOMODO', 'source_id': 'komodo.medium:255_8532', 'original_category': 'bacterial'}
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium255.pdf
Composition
flowchart LR
CultureMech_001354["clostridium_gs_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001354 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001354 --> ing1_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing2_Urea["Urea"]
CultureMech_001354 --> ing2_Urea
CHEBI_16199["CHEBI:16199"]
ing2_Urea -.-> CHEBI_16199
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001354 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001354 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001354 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Morpholinopropane_sulfonic_acid["Morpholinopropane sulfonic acid"]
CultureMech_001354 --> ing6_Morpholinopropane_sulfonic_acid
CHEBI_44115["CHEBI:44115"]
ing6_Morpholinopropane_sulfonic_acid -.-> CHEBI_44115
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001354 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Yeast_extract["Yeast extract"]
CultureMech_001354 --> ing8_Yeast_extract
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001354 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Cellobiose["Cellobiose"]
CultureMech_001354 --> ing10_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing10_Cellobiose -.-> CHEBI_17057
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.499002 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.998004 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
Urea
CH4N2O 60.056 g/mol
|
1.99601 G_PER_L |
CHEBI:16199
urea
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.499002 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0499002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0012475 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
Morpholinopropane sulfonic acid
C7H15NO4S 209.267 g/mol
|
9.98004 G_PER_L |
CHEBI:44115
3-(N-morpholino)propanesulfonic acid
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000499002 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 | Yeast extract | 5.98802 G_PER_L | ||
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.998004 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Cellobiose
C12H22O11 342.297 g/mol
|
4.99002 G_PER_L |
CHEBI:17057
cellobiose
|
Preparation
- Dissolve ingredients except cysteine and cellobiose and adjust pH to 7.2. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and readjust pH to 7.2, if necessary. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add cellobiose from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration.
Target organisms
No target organisms are recorded.
Provenance
Merged from 4 source records.
- KOMODO_255_GS_medium (CultureMech:004659)
- clostridium_gs_medium (CultureMech:001354)
- medium_255_modified_for_dsm_14427 (CultureMech:004657)
- medium_255_modified_for_dsm_8532 (CultureMech:004658)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.601371Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 255 |
| 2026-03-13T06:19:03.357773Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001354 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.275932+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM (GS) MEDIUM" to "clostridium_gs_medium" for consistent matching |
| 2026-06-05T07:40:53.910159+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.689487+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:46.230757+00:00 | recipe-merger | Merged 4 duplicate recipes into canonical record | Sources: KOMODO_255_GS_medium, clostridium_gs_medium, medium_255_modified_for_dsm_14427, medium_255_modified_for_dsm_8532 |
| 2026-08-06T03:04:46.230812+00:00 | merge_recipes.py | MERGED_RECIPES | merged 4 source recipe(s) on fingerprint a95122e6dde67d17873f3f53ac3c4ccf1c5efa72f88541a4823debb92b4a4d37 |