Overview
- External ontology
- komodo.medium:255 (source link unavailable) — GS medium
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: KOMODO ModelSEED | ID: 255 | DSMZ Medium: 255 (mediadive.medium:255) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004659["gs_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_004659 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_004659 --> ing1_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing2_Urea["Urea"]
CultureMech_004659 --> ing2_Urea
CHEBI_16199["CHEBI:16199"]
ing2_Urea -.-> CHEBI_16199
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_004659 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004659 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_004659 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Morpholinopropane_sulfonic_acid["Morpholinopropane sulfonic acid"]
CultureMech_004659 --> ing6_Morpholinopropane_sulfonic_acid
CHEBI_44115["CHEBI:44115"]
ing6_Morpholinopropane_sulfonic_acid -.-> CHEBI_44115
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_004659 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Yeast_extract["Yeast extract"]
CultureMech_004659 --> ing8_Yeast_extract
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_004659 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Cellobiose["Cellobiose"]
CultureMech_004659 --> ing10_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing10_Cellobiose -.-> CHEBI_17057
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.499002 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.998004 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
Urea
CH4N2O 60.056 g/mol
|
1.99601 G_PER_L |
CHEBI:16199
urea
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.499002 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0499002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0012475 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
Morpholinopropane sulfonic acid
C7H15NO4S 209.267 g/mol
|
9.98004 G_PER_L |
CHEBI:44115
3-(N-morpholino)propanesulfonic acid
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000499002 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 | Yeast extract | 5.98802 G_PER_L | ||
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.998004 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Cellobiose
C12H22O11 342.297 g/mol
|
4.99002 G_PER_L |
CHEBI:17057
cellobiose
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 255 |
| 2026-02-03T11:18:54.999124 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 255 |
| 2026-03-13T06:19:27.922421Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004659 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:43.227347+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GS medium" to "gs_medium" for consistent matching |
| 2026-06-05T07:40:21.918736+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.455882+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |