pyrobaculum_neutrophilum_medium

Overview

External ontology

mediadive.medium:291 (source link unavailable) — PYROBACULUM NEUTROPHILUM MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium291.pdf

Composition

flowchart LR
    CultureMech_001390["pyrobaculum_neutrophilum_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001390 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001390 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001390 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001390 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001390 --> ing4_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing5_Sulfur["Sulfur"]
    CultureMech_001390 --> ing5_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing5_Sulfur -.-> CHEBI_26833
    ing6_Resazurin["Resazurin"]
    CultureMech_001390 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001390 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001390 --> ing8_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing8_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001390 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001390 --> ing10_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001390 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_001390 --> ing12_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing12_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001390 --> ing13_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001390 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (15)

Preparation

1. Adjust pH to 6.5 with NaOH, bring to boil for 5 min, then cool to room temperature under H2 + CO2 (80% + 20%) gas mixture. Add 0.85 g NaHCO3 per liter and equilibrate the pH to c. 6.5 by further gassing.
2. Dispense in serum bottles (10 ml/30 ml bottle), seal and heat the bottles for one hour at 85°C on each of three successive days.
3. Before inoculation, add from anaerobic stock solutions 0.02% yeast extract (for mixotrophic growth only) and neutralized Na2S x 9 H2O solution, 10% (w/v). After inoculation, pressurize to 1 bar H2 + CO2 overpressure.
4. Prepare medium, then add per liter:
5. Adjust pH of final solution to 2 with 1 N HCl.
6. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• pyrobaculum_neutrophilum_medium (CultureMech:001390)