pyrobaculum_neutrophilum_medium

CultureMech:001390 archaea DEFINED LIQUID
Original name: PYROBACULUM NEUTROPHILUM MEDIUM

Overview

External ontology
mediadive.medium:291 (source link unavailable) — PYROBACULUM NEUTROPHILUM MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium291.pdf

Composition

flowchart LR
    CultureMech_001390["pyrobaculum_neutrophilum_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001390 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001390 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001390 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001390 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001390 --> ing4_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing5_Sulfur["Sulfur"]
    CultureMech_001390 --> ing5_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing5_Sulfur -.-> CHEBI_26833
    ing6_Resazurin["Resazurin"]
    CultureMech_001390 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001390 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001390 --> ing8_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing8_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001390 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001390 --> ing10_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001390 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_001390 --> ing12_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing12_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001390 --> ing13_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001390 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.02 G_PER_L CHEBI:86254
iron trichloride hexahydrate
6 Sulfur
S 32.067 g/mol
8 G_PER_L CHEBI:26833
sulfur atom
7 Resazurin
C12H7NO4 229.191 g/mol
0.0004 G_PER_L CHEBI:8806
Resazurin
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.18 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.45 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.005 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
0.003 G_PER_L CHEBI:87009
vanadyl sulfate dihydrate
14 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.001 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
15 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Adjust pH to 6.5 with NaOH, bring to boil for 5 min, then cool to room temperature under H2 + CO2 (80% + 20%) gas mixture. Add 0.85 g NaHCO3 per liter and equilibrate the pH to c. 6.5 by further gassing.
  2. Dispense in serum bottles (10 ml/30 ml bottle), seal and heat the bottles for one hour at 85°C on each of three successive days.
  3. Before inoculation, add from anaerobic stock solutions 0.02% yeast extract (for mixotrophic growth only) and neutralized Na2S x 9 H2O solution, 10% (w/v). After inoculation, pressurize to 1 bar H2 + CO2 overpressure.
  4. Prepare medium, then add per liter:
  5. Adjust pH of final solution to 2 with 1 N HCl.
  6. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.679835Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 291
2026-03-13T06:19:03.666663Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001390
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.623619+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYROBACULUM NEUTROPHILUM MEDIUM" to "pyrobaculum_neutrophilum_medium" for consistent matching
2026-06-05T07:41:43.259392+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.651453+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.