bsk_medium

Overview

External ontology

mediadive.medium:403 (source link unavailable) — BSK-MEDIUM

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium403.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001513["bsk_medium"]
    ing0_CMRL_1066["CMRL 1066"]
    CultureMech_001513 --> ing0_CMRL_1066
    ing1_Proteose_peptone_no__2["Proteose peptone no. 2"]
    CultureMech_001513 --> ing1_Proteose_peptone_no__2
    ing2_Tryptone_peptone["Tryptone peptone"]
    CultureMech_001513 --> ing2_Tryptone_peptone
    ing3_Proteose_yeastolate["Proteose yeastolate"]
    CultureMech_001513 --> ing3_Proteose_yeastolate
    ing4_HEPES_buffer["HEPES buffer"]
    CultureMech_001513 --> ing4_HEPES_buffer
    CHEBI_46756["CHEBI:46756"]
    ing4_HEPES_buffer -.-> CHEBI_46756
    ing5_Sodium_citrate["Sodium citrate"]
    CultureMech_001513 --> ing5_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing5_Sodium_citrate -.-> CHEBI_53258
    ing6_Glucose["Glucose"]
    CultureMech_001513 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Na_pyruvate["Na-pyruvate"]
    CultureMech_001513 --> ing7_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing7_Na_pyruvate -.-> CHEBI_50144
    ing8_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_001513 --> ing8_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing8_N_Acetylglucosamine -.-> CHEBI_59640
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001513 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_L_Glutamine["L-Glutamine"]
    CultureMech_001513 --> ing10_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing10_L_Glutamine -.-> CHEBI_18050
    ing11_Double_distilled_water["Double distilled water"]
    CultureMech_001513 --> ing11_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Double_distilled_water -.-> CHEBI_15377
    ing12_Gelatine["Gelatine"]
    CultureMech_001513 --> ing12_Gelatine
    CHEBI_5291["CHEBI:5291"]
    ing12_Gelatine -.-> CHEBI_5291
    ing13_Tryptone["Tryptone"]
    CultureMech_001513 --> ing13_Tryptone
    ing14_Yeast_extract["Yeast extract"]
    CultureMech_001513 --> ing14_Yeast_extract
    ing15_Sodium_chloride["Sodium chloride"]
    CultureMech_001513 --> ing15_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing15_Sodium_chloride -.-> CHEBI_26710
    ing16_Rabbit_serum["Rabbit serum"]
    CultureMech_001513 --> ing16_Rabbit_serum

Ingredients (17)

Preparation

1. Combine the warm (37°C) solutions A, B, C, D, and filter-sterilize the warm medium. Solution B can also be added after filtration.
2. Alternatively, buy supplemented or un-supplemented BSK-H medium (Fa. Bio&SELL or Sigma) and supplement the medium according to manufacturer's advice. Sterilize the mixed and warm (37°C) medium by filtration.
3. Stir slowly at 4 - 10°C for 3 hours, adjust pH to 7.6 with 5 M NaOH. Filter sterilize.
4. Dissolve 14.0 g gelatine in 200 ml bidest. water, autoclave 15 minutes at 115°C.
5. Dissolve 50.05 g bovine serum albumine, fract. V (important: Sigma No A9647) in 143 ml bidest. water, or use BSA-solution Sigma A7409.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• bsk_medium (CultureMech:001513)