Overview
- External ontology
- mediadive.medium:403 (source link unavailable) — BSK-MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium403.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The LB constituent expansion is withdrawn: the exact DSMZ formulation specifies bovine serum albumin instead. Source batch, stock and receiving-medium scopes are separate; chemical groundings and growth claims are not recertified. Source alternative, not an extra preparation step: Alternatively, buy supplemented or un-supplemented BSK-H medium (Fa. Bio&SELL or Sigma) and supplement the medium according to manufacturer's advice. Sterilize the mixed and warm (37°C) medium by filtration.
Ingredients (0)
No ingredients recorded.
Preparation
- Combine the warm (37°C) solutions A, B, C, D, and filter-sterilize the warm medium. Solution B can also be added after filtration.
Solutions
Solution A
Dose: 1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Proteose peptone no. 2 | 5 G_PER_L | Source batch input: 5 g. Retain provider-declared stock g_l=5 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. Source qualifier: DIFCO 0121-01-3. |
| Tryptone peptone | 1 G_PER_L | Source batch input: 1 g. Retain provider-declared stock g_l=1 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. Source qualifier: DIFCO 0123-01. |
| Proteose yeastolate | 1 G_PER_L | Source batch input: 1 g. Retain provider-declared stock g_l=1 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. Source qualifier: TC) (DIFCO 5577-15. |
| HEPES buffer | 6 G_PER_L | Source batch input: 6 g. Retain provider-declared stock g_l=6 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. Source qualifier: acid) (SIGMA H3375. |
| Sodium citrate | 0.7 G_PER_L | Source batch input: 0.7 g. Retain provider-declared stock g_l=0.7 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. |
| Glucose | 3 G_PER_L | Source batch input: 3 g. Retain provider-declared stock g_l=3 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. Source qualifier: alpha-, D+. |
| Na-pyruvate | 0.8 G_PER_L | Source batch input: 0.8 g. Retain provider-declared stock g_l=0.8 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. |
| N-Acetylglucosamine | 0.4 G_PER_L | Source batch input: 0.4 g. Retain provider-declared stock g_l=0.4 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. |
| MgCl2 x 6 H2O | 0.3 G_PER_L | Source batch input: 0.3 g. Retain provider-declared stock g_l=0.3 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. |
| L-Glutamine | 0.1 G_PER_L | Source batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only within Solution A, not a receiving-medium concentration or independently measured final stock volume. |
| Double distilled water | 0.9 L | [Merged 3 duplicates: 900.0, 200.0, 143.0] Source water batch input: 900 ml / 1000 = 0.9 L in this preparation only, not a measured final volume. Earlier duplicate-collapse notes describe the superseded flattened record; water is now split between preparations A, B and C. Source qualifier: filter-sterilized. |
Source batch dose: 1000 ml / 1000 = 1 L per receiving batch, not g/L or a per-liter dose.
CMRL 1066
Dose: 0.1 L
Source batch dose: 100 ml / 1000 = 0.1 L per receiving batch, not g/L or a per-liter dose. Source product/stock qualifier: GIBCO 042-1545, glutamin 10 x.
NaOH solution (5 M)
| Ingredient | Concentration | Notes |
|---|---|---|
| NaOH | 5 MOLAR | Explicit 5 M NaOH titrant strength for adjusting Solution A to pH 7.6, not a receiving-medium concentration. No fixed titrant dose is specified. |
Explicit 5 M NaOH titrant strength for adjusting Solution A to pH 7.6, not a receiving-medium concentration. No fixed titrant dose is specified.
Solution B
Dose: 0.2 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Gelatine | 70 G_PER_L | Source batch input: 14 g. Retain provider-declared stock g_l=70 only within Solution B, not a receiving-medium concentration or independently measured final stock volume. |
| Double distilled water | 0.2 L | Source water batch input: 200 ml / 1000 = 0.2 L in this preparation only, not a measured final volume. |
Source batch dose: 200 ml / 1000 = 0.2 L per receiving batch, not g/L or a per-liter dose.
Solution C
Dose: 0.143 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Bovine serum albumin | 350 G_PER_L | Source batch input: 50.05 g. Retain provider-declared stock g_l=350 only within Solution C, not a receiving-medium concentration or independently measured final stock volume. Source qualifier: Sigma No A9647. |
| Double distilled water | 0.143 L | Source water batch input: 143 ml / 1000 = 0.143 L in this preparation only, not a measured final volume. |
Source batch dose: 143 ml / 1000 = 0.143 L per receiving batch, not g/L or a per-liter dose.
Rabbit serum
Dose: 0.086 L
Source batch dose: 86 ml / 1000 = 0.086 L per receiving batch, not g/L or a per-liter dose. Source product/stock qualifier: inactivated, GIBCO 037-06120M.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:11.055344Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 403 |
| 2026-03-13T03:09:08.053416Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:04.841594Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001513 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.910866+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BSK-MEDIUM" to "bsk_medium" for consistent matching |
| 2026-06-05T07:40:51.670850+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.989064+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T22:13:10.504331+00:00 | codex | RESTORED_DSMZ_ALBUMIN_PREPARATIONS_AND_CONCENTRATION_SCOPES_WITH_EVIDENCE | Restore source-specific albumin, withdraw false LB inputs, separate preparation doses and stock compositions, and attach exact evidence for changed claims. Preserve IDs, unrelated groundings and earlier history. Unknown percentage bases and final-medium volume remain unknown. |