ectothiorhodospira_abdelmalekii_medium

CultureMech:001539 bacterial DEFINED LIQUID
Original name: ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM

Overview

External ontology
mediadive.medium:430 (source link unavailable) — ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'medium_430_modified_for_dsm_13718', 'source': 'KOMODO', 'source_id': 'komodo.medium:430_13718', 'original_category': 'bacterial'}
pH
8.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium430.pdf

Composition

flowchart LR
    CultureMech_001539["ectothiorhodospira_abdelmalekii_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001539 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001539 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001539 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001539 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_NaCl["NaCl"]
    CultureMech_001539 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Na2SO4["Na2SO4"]
    CultureMech_001539 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_Na_acetate["Na-acetate"]
    CultureMech_001539 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na2CO3["Na2CO3"]
    CultureMech_001539 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_NaHCO3["NaHCO3"]
    CultureMech_001539 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001539 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001539 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001539 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001539 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001539 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_ZnCl2["ZnCl2"]
    CultureMech_001539 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_H3BO3["H3BO3"]
    CultureMech_001539 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001539 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001539 --> ing17_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing18_Biotin["Biotin"]
    CultureMech_001539 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_Nicotinic_acid_amide["Nicotinic acid amide"]
    CultureMech_001539 --> ing19_Nicotinic_acid_amide
    CHEBI_17154["CHEBI:17154"]
    ing19_Nicotinic_acid_amide -.-> CHEBI_17154
    ing20_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001539 --> ing20_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing20_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001539 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
    CultureMech_001539 --> ing22_Pyridoxal_hydrochloride
    CHEBI_131529["CHEBI:131529"]
    ing22_Pyridoxal_hydrochloride -.-> CHEBI_131529
    ing23_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001539 --> ing23_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_001539 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001539 --> ing25_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing25_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.8 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.8 G_PER_L CHEBI:31206
ammonium chloride
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 NaCl
Cl.Na 58.443 g/mol
120 G_PER_L CHEBI:26710
sodium chloride
6 Na2SO4
2Na.O4S 142.043 g/mol
15 G_PER_L CHEBI:32149
sodium sulfate
7 Na-acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
8 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
 (adjust if required)
9 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.8 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.25 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.07 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
16 H3BO3
H3BO3 61.833 g/mol
0.5 G_PER_L CHEBI:33118
boric acid
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.01 G_PER_L CHEBI:131361
disodium selenite pentahydrate
19 Biotin
C10H16N2O3S 244.316 g/mol
0.1 G_PER_L CHEBI:15956
biotin
20 Nicotinic acid amide
C6H6N2O 122.127 g/mol
0.35 G_PER_L CHEBI:17154
nicotinamide
21 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.3 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.2 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
0.1 G_PER_L CHEBI:131529
pyridoxal hydrochloride
24 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
25 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
26 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
100 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Dissolve and bring to the boil.
  2. Allow to cool down to room temperature under N2 gas phase. Then add bicarbonate.
  3. Adjust pH to 8.5.
  4. Dispense into appropriate vessels, e.g. Hungate tubes, under N2 atmosphere.
  5. Autoclave at 121 °C for 15 min.
  6. After autoclaving add sterile-filtered vitamin and sulfide solutions.
  7. Adjust pH to 2.0 - 3.0.
  8. The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:11.115336Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 430
2026-03-13T06:19:05.049020Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001539
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.133772+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM" to "ectothiorhodospira_abdelmalekii_medium" for consistent matching
2026-06-05T07:40:59.309333+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.615636+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:33.345026+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: ectothiorhodospira_abdelmalekii_medium, medium_430_modified_for_dsm_13718
2026-08-06T03:05:33.345069+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 828e5ee624aeb252227d9f60ae3c74c0ebd510354bd5a41b2b342bbeadeae471