ectothiorhodospira_abdelmalekii_medium

CultureMech:001539 bacterial DEFINED LIQUID
Original name: ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM

Overview

External ontology
mediadive.medium:430 (source link unavailable) — ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM
Applications
  • Microbial cultivation
pH
8.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium430.pdf

Composition

flowchart LR
    CultureMech_001539["ectothiorhodospira_abdelmalekii_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001539 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001539 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001539 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001539 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_NaCl["NaCl"]
    CultureMech_001539 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Na2SO4["Na2SO4"]
    CultureMech_001539 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_Na_acetate["Na-acetate"]
    CultureMech_001539 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na2CO3["Na2CO3"]
    CultureMech_001539 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_NaHCO3["NaHCO3"]
    CultureMech_001539 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001539 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001539 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001539 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001539 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_ZnCl2["ZnCl2"]
    CultureMech_001539 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_H3BO3["H3BO3"]
    CultureMech_001539 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001539 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001539 --> ing16_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing17_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
    CultureMech_001539 --> ing17_Pyridoxal_hydrochloride
    CHEBI_131529["CHEBI:131529"]
    ing17_Pyridoxal_hydrochloride -.-> CHEBI_131529
    ing18_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001539 --> ing18_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_Calcium_pantothenate -.-> CHEBI_31345
    ing19_Vitamin_B12["Vitamin B12"]
    CultureMech_001539 --> ing19_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing19_Vitamin_B12 -.-> CHEBI_176843
    ing20_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001539 --> ing20_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing20_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.8 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.8 G_PER_L CHEBI:31206
ammonium chloride
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 NaCl
Cl.Na 58.443 g/mol
120 G_PER_L CHEBI:26710
sodium chloride
6 Na2SO4
2Na.O4S 142.043 g/mol
15 G_PER_L CHEBI:32149
sodium sulfate
7 Na-acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
8 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
 (adjust if required)
9 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.25 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.07 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
15 H3BO3
H3BO3 61.833 g/mol
0.5 G_PER_L CHEBI:33118
boric acid
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.01 G_PER_L CHEBI:131361
disodium selenite pentahydrate
18 Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
0.1 G_PER_L CHEBI:131529
pyridoxal hydrochloride
19 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
20 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
21 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
100 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Dissolve and bring to the boil.
  2. Allow to cool down to room temperature under N2 gas phase. Then add bicarbonate.
  3. Adjust pH to 8.5.
  4. Dispense into appropriate vessels, e.g. Hungate tubes, under N2 atmosphere.
  5. Autoclave at 121 °C for 15 min.
  6. After autoclaving add sterile-filtered vitamin and sulfide solutions.
  7. Adjust pH to 2.0 - 3.0.
  8. The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Solutions

Trace element solution (SLA)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.8 G_PER_L

Vitamin solution (VA)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 0.1 G_PER_L
Nicotinic acid amide 0.35 G_PER_L
Thiamine-HCl x 2 H2O 0.3 G_PER_L
p-Aminobenzoic acid 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.115336Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 430
2026-03-13T06:19:05.049020Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001539
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.133772+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM" to "ectothiorhodospira_abdelmalekii_medium" for consistent matching
2026-06-05T07:40:59.309333+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.615636+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.097900+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution (SLA)' @ 1 ml/l; 4 -> 'Vitamin solution (VA)' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).