Overview
- External ontology
- mediadive.medium:431 (source link unavailable) — ECTOTHIORHODOSPIRA VACUOLATA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.7
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium431.pdf
Composition
flowchart LR
CultureMech_001540["ectothiorhodospira_vacuolata_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001540 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_001540 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001540 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001540 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaHCO3["NaHCO3"]
CultureMech_001540 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_NaCl["NaCl"]
CultureMech_001540 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001540 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001540 --> ing7_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing8_Na_malate["Na-malate"]
CultureMech_001540 --> ing8_Na_malate
CHEBI_91260["CHEBI:91260"]
ing8_Na_malate -.-> CHEBI_91260
ing9_Biotin["Biotin"]
CultureMech_001540 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Nicotinic_acid_amide["Nicotinic acid amide"]
CultureMech_001540 --> ing10_Nicotinic_acid_amide
CHEBI_17154["CHEBI:17154"]
ing10_Nicotinic_acid_amide -.-> CHEBI_17154
ing11_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001540 --> ing11_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing11_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001540 --> ing12_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
ing13_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
CultureMech_001540 --> ing13_Pyridoxal_hydrochloride
CHEBI_131529["CHEBI:131529"]
ing13_Pyridoxal_hydrochloride -.-> CHEBI_131529
ing14_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001540 --> ing14_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing14_Calcium_pantothenate -.-> CHEBI_31345
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_001540 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001540 --> ing16_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001540 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001540 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001540 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001540 --> ing20_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
ing21_ZnCl2["ZnCl2"]
CultureMech_001540 --> ing21_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing21_ZnCl2 -.-> CHEBI_49976
ing22_H3BO3["H3BO3"]
CultureMech_001540 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001540 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001540 --> ing24_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.05 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 9 | Na-malate | 1 G_PER_L |
CHEBI:91260
disodium malate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Nicotinic acid amide
C6H6N2O 122.127 g/mol
|
0.35 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 12 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.3 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 13 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.2 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 14 |
Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
|
0.1 G_PER_L |
CHEBI:131529
pyridoxal hydrochloride
|
|
| 15 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 16 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 17 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.8 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.25 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.07 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 22 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.01 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Adjust pH to 8.7, filter sterilize and add appropriate amount of Na2S solution.
- Adjust pH to 2.0 - 3.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ectothiorhodospira_vacuolata_medium (CultureMech:001540)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.119647Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 431 |
| 2026-03-13T06:19:05.061242Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001540 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.147301+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ECTOTHIORHODOSPIRA VACUOLATA MEDIUM" to "ectothiorhodospira_vacuolata_medium" for consistent matching |
| 2026-06-05T07:40:59.348360+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.631820+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:33.389672+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ca0fc9ca6766eedab937c9d636bac81402b606eea23e2da38e16e11a0d30be9e |