ectothiorhodospira_vacuolata_medium

CultureMech:001540 bacterial DEFINED LIQUID
Original name: ECTOTHIORHODOSPIRA VACUOLATA MEDIUM

Overview

External ontology
mediadive.medium:431 (source link unavailable) — ECTOTHIORHODOSPIRA VACUOLATA MEDIUM
Applications
  • Microbial cultivation
pH
8.7
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium431.pdf

Composition

flowchart LR
    CultureMech_001540["ectothiorhodospira_vacuolata_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001540 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001540 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001540 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001540 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaHCO3["NaHCO3"]
    CultureMech_001540 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_NaCl["NaCl"]
    CultureMech_001540 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001540 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001540 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_Na_malate["Na-malate"]
    CultureMech_001540 --> ing8_Na_malate
    CHEBI_91260["CHEBI:91260"]
    ing8_Na_malate -.-> CHEBI_91260
    ing9_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
    CultureMech_001540 --> ing9_Pyridoxal_hydrochloride
    CHEBI_131529["CHEBI:131529"]
    ing9_Pyridoxal_hydrochloride -.-> CHEBI_131529
    ing10_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001540 --> ing10_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing10_Calcium_pantothenate -.-> CHEBI_31345
    ing11_Vitamin_B12["Vitamin B12"]
    CultureMech_001540 --> ing11_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing11_Vitamin_B12 -.-> CHEBI_176843
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001540 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001540 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001540 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001540 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_ZnCl2["ZnCl2"]
    CultureMech_001540 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_H3BO3["H3BO3"]
    CultureMech_001540 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001540 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001540 --> ing19_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NaHCO3
CHO3.Na 84.006 g/mol
3 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.05 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.5 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 Na-malate 1 G_PER_L CHEBI:91260
disodium malate
10 Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
0.1 G_PER_L CHEBI:131529
pyridoxal hydrochloride
11 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
12 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.25 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.07 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
18 H3BO3
H3BO3 61.833 g/mol
0.5 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.01 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Adjust pH to 8.7, filter sterilize and add appropriate amount of Na2S solution.
  2. Adjust pH to 2.0 - 3.0.

Solutions

Vitamin solution (VA)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 0.1 G_PER_L
Nicotinic acid amide 0.35 G_PER_L
Thiamine-HCl x 2 H2O 0.3 G_PER_L
p-Aminobenzoic acid 0.2 G_PER_L

Trace element solution (SLA)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.8 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.119647Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 431
2026-03-13T06:19:05.061242Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001540
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.147301+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ECTOTHIORHODOSPIRA VACUOLATA MEDIUM" to "ectothiorhodospira_vacuolata_medium" for consistent matching
2026-06-05T07:40:59.348360+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.631820+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.138349+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 4 -> 'Vitamin solution (VA)' @ 1 ml/l; 1 -> 'Trace element solution (SLA)' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).