medium_514_plus_additional_salt

CultureMech:001646 bacterial COMPLEX SOLID_AGAR
Original name: MEDIUM 514 plus additional salt

Overview

External ontology
mediadive.medium:514b (source link unavailable) — MEDIUM 514 plus additional salt
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'half_strength_bacto_marine_broth', 'source': 'MediaDive', 'source_id': 'mediadive.medium:514a', 'original_category': 'specialized'}
pH
7.6
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514b.pdf

Composition

flowchart LR
    CultureMech_001646["medium_514_plus_additional_salt"]
    ing0_Agar["Agar"]
    CultureMech_001646 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_Bacto_peptone["Bacto peptone"]
    CultureMech_001646 --> ing1_Bacto_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001646 --> ing2_Yeast_extract
    ing3_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_001646 --> ing3_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing3_Fe_III__citrate -.-> CHEBI_144421
    ing4_NaCl["NaCl"]
    CultureMech_001646 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MgCl2["MgCl2"]
    CultureMech_001646 --> ing5_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing5_MgCl2 -.-> CHEBI_6636
    ing6_Na2SO4["Na2SO4"]
    CultureMech_001646 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_CaCl2["CaCl2"]
    CultureMech_001646 --> ing7_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing7_CaCl2 -.-> CHEBI_3312
    ing8_KCl["KCl"]
    CultureMech_001646 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NaHCO3["NaHCO3"]
    CultureMech_001646 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_KBr["KBr"]
    CultureMech_001646 --> ing10_KBr
    CHEBI_32030["CHEBI:32030"]
    ing10_KBr -.-> CHEBI_32030
    ing11_SrCl2["SrCl2"]
    CultureMech_001646 --> ing11_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing11_SrCl2 -.-> CHEBI_36383
    ing12_H3BO3["H3BO3"]
    CultureMech_001646 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_Na_silicate["Na-silicate"]
    CultureMech_001646 --> ing13_Na_silicate
    CHEBI_60720["CHEBI:60720"]
    ing13_Na_silicate -.-> CHEBI_60720
    ing14_NaF["NaF"]
    CultureMech_001646 --> ing14_NaF
    CHEBI_28741["CHEBI:28741"]
    ing14_NaF -.-> CHEBI_28741
    ing15__NH4_NO3["(NH4)NO3"]
    CultureMech_001646 --> ing15__NH4_NO3
    CHEBI_63038["CHEBI:63038"]
    ing15__NH4_NO3 -.-> CHEBI_63038
    ing16_Na2HPO4["Na2HPO4"]
    CultureMech_001646 --> ing16_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing16_Na2HPO4 -.-> CHEBI_34683

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Agar 17.5 G_PER_L CHEBI:2509
agar
 (for solid medium)
2 Bacto peptone 5 G_PER_L
3 Yeast extract 1 G_PER_L
4 Fe(III) citrate
C6H5FeO7 244.944 g/mol
0.1 G_PER_L CHEBI:144421
iron(III) citrate
5 NaCl
Cl.Na 58.443 g/mol
19.45 G_PER_L CHEBI:26710
sodium chloride
6 MgCl2
2Cl.Mg 95.211 g/mol
5.9 G_PER_L CHEBI:6636
magnesium dichloride
7 Na2SO4
2Na.O4S 142.043 g/mol
3.24 G_PER_L CHEBI:32149
sodium sulfate
8 CaCl2
Ca.2Cl 110.984 g/mol
1.8 G_PER_L CHEBI:3312
calcium dichloride
9 KCl
Cl.K 74.551 g/mol
0.55 G_PER_L CHEBI:32588
potassium chloride
10 NaHCO3
CHO3.Na 84.006 g/mol
0.16 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 KBr
Br.K 119.002 g/mol
0.08 G_PER_L CHEBI:32030
potassium bromide
12 SrCl2
2Cl.Sr 158.526 g/mol
0.034 G_PER_L CHEBI:36383
strontium dichloride
13 H3BO3
H3BO3 61.833 g/mol
0.022 G_PER_L CHEBI:33118
boric acid
14 Na-silicate
2Na.O3Si 122.063 g/mol
0.004 G_PER_L CHEBI:60720
sodium silicate
15 NaF
F.Na 41.988 g/mol
0.0024 G_PER_L CHEBI:28741
sodium fluoride
16 (NH4)NO3
H4N.NO3 80.043 g/mol
0.0016 G_PER_L CHEBI:63038
ammonium nitrate
17 Na2HPO4
HO4P.2Na 141.958 g/mol
0.008 G_PER_L CHEBI:34683
disodium hydrogenphosphate

Preparation

  1. Use medium 514 to which is added NaCl at the concentration indicated (i.e. "514 + 6% NaCl" indicates use medium 514 to which 60 g/l NaCl has been added; see strain dependant modifications).
  2. Final pH 7.6. The medium may be solidified by adding 15 - 20 g/l agar.
  3. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.
  4. Note: M.514 refers to Bacto Marine Broth Difco 2216 (BD 279110). Other brands (even if they refer to Difco2216) have not been tested and might not support growth for some strains.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:11.481945Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 514b
2026-03-13T06:19:06.144490Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001646
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.311596+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM 514 plus additional salt" to "medium_514_plus_additional_salt" for consistent matching
2026-06-05T07:41:24.826884+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:04.861675+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:42.714482+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: medium_514_plus_additional_salt, half_strength_bacto_marine_broth
2026-08-06T03:05:42.714522+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 005b39953fdbd385c2718556c26c34ed69db2e7a4a3e7352a6fe7f68052d5178