Overview
- External ontology
- mediadive.medium:514b (source link unavailable) — MEDIUM 514 plus additional salt
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514b.pdf
Composition
flowchart LR
CultureMech_001646["medium_514_plus_additional_salt"]
ing0_Agar["Agar"]
CultureMech_001646 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1_Bacto_peptone["Bacto peptone"]
CultureMech_001646 --> ing1_Bacto_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_001646 --> ing2_Yeast_extract
ing3_Fe_III__citrate["Fe(III) citrate"]
CultureMech_001646 --> ing3_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing3_Fe_III__citrate -.-> CHEBI_144421
ing4_NaCl["NaCl"]
CultureMech_001646 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgCl2["MgCl2"]
CultureMech_001646 --> ing5_MgCl2
CHEBI_6636["CHEBI:6636"]
ing5_MgCl2 -.-> CHEBI_6636
ing6_Na2SO4["Na2SO4"]
CultureMech_001646 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_CaCl2["CaCl2"]
CultureMech_001646 --> ing7_CaCl2
CHEBI_3312["CHEBI:3312"]
ing7_CaCl2 -.-> CHEBI_3312
ing8_KCl["KCl"]
CultureMech_001646 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaHCO3["NaHCO3"]
CultureMech_001646 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_KBr["KBr"]
CultureMech_001646 --> ing10_KBr
CHEBI_32030["CHEBI:32030"]
ing10_KBr -.-> CHEBI_32030
ing11_SrCl2["SrCl2"]
CultureMech_001646 --> ing11_SrCl2
CHEBI_36383["CHEBI:36383"]
ing11_SrCl2 -.-> CHEBI_36383
ing12_H3BO3["H3BO3"]
CultureMech_001646 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_Na_silicate["Na-silicate"]
CultureMech_001646 --> ing13_Na_silicate
CHEBI_60720["CHEBI:60720"]
ing13_Na_silicate -.-> CHEBI_60720
ing14_NaF["NaF"]
CultureMech_001646 --> ing14_NaF
CHEBI_28741["CHEBI:28741"]
ing14_NaF -.-> CHEBI_28741
ing15__NH4_NO3["(NH4)NO3"]
CultureMech_001646 --> ing15__NH4_NO3
CHEBI_63038["CHEBI:63038"]
ing15__NH4_NO3 -.-> CHEBI_63038
ing16_Na2HPO4["Na2HPO4"]
CultureMech_001646 --> ing16_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing16_Na2HPO4 -.-> CHEBI_34683
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 17.5 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 2 | Bacto peptone | 5 G_PER_L | ||
| 3 | Yeast extract | 1 G_PER_L | ||
| 4 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.1 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
19.45 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
MgCl2
2Cl.Mg 95.211 g/mol
|
5.9 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.24 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1.8 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.55 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
KBr
Br.K 119.002 g/mol
|
0.08 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 12 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.034 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.022 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
Na-silicate
2Na.O3Si 122.063 g/mol
|
0.004 G_PER_L |
CHEBI:60720
sodium silicate
|
|
| 15 |
NaF
F.Na 41.988 g/mol
|
0.0024 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 16 |
(NH4)NO3
H4N.NO3 80.043 g/mol
|
0.0016 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 17 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.008 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Preparation
- Use medium 514 to which is added NaCl at the concentration indicated (i.e. "514 + 6% NaCl" indicates use medium 514 to which 60 g/l NaCl has been added; see strain dependant modifications).
- Final pH 7.6. The medium may be solidified by adding 15 - 20 g/l agar.
- Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.
- Note: M.514 refers to Bacto Marine Broth Difco 2216 (BD 279110). Other brands (even if they refer to Difco2216) have not been tested and might not support growth for some strains.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.481945Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 514b |
| 2026-03-13T06:19:06.144490Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001646 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.311596+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 514 plus additional salt" to "medium_514_plus_additional_salt" for consistent matching |
| 2026-06-05T07:41:24.826884+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:04.861675+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |