Overview
- External ontology
- mediadive.medium:520 (source link unavailable) — RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'cm3_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:520', 'original_category': 'bacterial'}
- {'name': 'for_dsm_5974_and_dsm_17427', 'source': 'KOMODO', 'source_id': 'komodo.medium:520.1', 'original_category': 'bacterial'}
- {'name': 'medium_520_modified_for_dsm_7093', 'source': 'KOMODO', 'source_id': 'komodo.medium:520_7093', 'original_category': 'bacterial'}
- {'name': 'medium_520_modified_for_dsm_9801', 'source': 'KOMODO', 'source_id': 'komodo.medium:520_9801', 'original_category': 'bacterial'}
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium520.pdf
Composition
flowchart LR
CultureMech_001653["ruminoclostridium_cellulolyticum_cm3_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_001653 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_001653 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001653 --> ing2_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001653 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_Yeast_extract["Yeast extract"]
CultureMech_001653 --> ing4_Yeast_extract
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_001653 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001653 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001653 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_Cellobiose["Cellobiose"]
CultureMech_001653 --> ing8_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing8_Cellobiose -.-> CHEBI_17057
ing9_Cellulose["Cellulose"]
CultureMech_001653 --> ing9_Cellulose
CHEBI_18246["CHEBI:18246"]
ing9_Cellulose -.-> CHEBI_18246
ing10_Na2CO3["Na2CO3"]
CultureMech_001653 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001653 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_HCl["HCl"]
CultureMech_001653 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001653 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_ZnCl2["ZnCl2"]
CultureMech_001653 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001653 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_H3BO3["H3BO3"]
CultureMech_001653 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001653 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001653 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001653 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001653 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.29611 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.49551 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
2.89133 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00124626 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 5 | Yeast extract | 1.99402 G_PER_L | ||
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000498504 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.199402 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0747757 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
Cellobiose
C12H22O11 342.297 g/mol
|
5.98205 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 10 |
Cellulose
(C6H10O5)n.H2O 180.156 g/mol
|
9.97009 G_PER_L |
CHEBI:18246
(1->4)-beta-D-glucan
|
Optional ingredient (MN 301) |
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.49551 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.498504 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Dissolve ingredients except magnesium chloride, calcium chloride, cellobiose, cysteine and carbonate, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add magnesium chloride, calcium chloride and cellobiose from anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Cellobiose has to be sterilized by filtration. Prior to inoculation add cysteine from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH to 7.2.
- Note: Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose powder MN 301 (MACHEREY-NAGEL).
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 5 source records.
- KOMODO_520_CM3_medium (CultureMech:005882)
- for_dsm_5974_and_dsm_17427 (CultureMech:005879)
- medium_520_modified_for_dsm_7093 (CultureMech:005880)
- medium_520_modified_for_dsm_9801 (CultureMech:005881)
- ruminoclostridium_cellulolyticum_cm3_medium (CultureMech:001653)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.511727Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 520 |
| 2026-03-13T06:19:06.215318Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001653 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.388940+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM" to "ruminoclostridium_cellulolyticum_cm3_medium" for consistent matching |
| 2026-06-05T07:41:44.850221+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.149844+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:55.065739+00:00 | recipe-merger | Merged 5 duplicate recipes into canonical record | Sources: KOMODO_520_CM3_medium, for_dsm_5974_and_dsm_17427, medium_520_modified_for_dsm_7093, medium_520_modified_for_dsm_9801, ruminoclostridium_cellulolyticum_cm3_medium |
| 2026-08-06T03:04:55.065806+00:00 | merge_recipes.py | MERGED_RECIPES | merged 5 source recipe(s) on fingerprint ddd6dbc12d273e4ce333556f40136dbf51ecca9a27077396a1706326a048a79e |