ruminoclostridium_cellulolyticum_cm3_medium

CultureMech:001653 bacterial COMPLEX LIQUID
Original name: RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM

Overview

External ontology
mediadive.medium:520 (source link unavailable) — RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium520.pdf

Composition

flowchart LR
    CultureMech_001653["ruminoclostridium_cellulolyticum_cm3_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001653 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001653 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001653 --> ing2_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001653 --> ing3_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001653 --> ing4_Yeast_extract
    ing5_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001653 --> ing5_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Sodium_resazurin -.-> CHEBI_8806
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001653 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001653 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Cellobiose["Cellobiose"]
    CultureMech_001653 --> ing8_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing8_Cellobiose -.-> CHEBI_17057
    ing9_Cellulose["Cellulose"]
    CultureMech_001653 --> ing9_Cellulose
    CHEBI_18246["CHEBI:18246"]
    ing9_Cellulose -.-> CHEBI_18246
    ing10_Na2CO3["Na2CO3"]
    CultureMech_001653 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001653 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_HCl["HCl"]
    CultureMech_001653 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001653 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_ZnCl2["ZnCl2"]
    CultureMech_001653 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001653 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_001653 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001653 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001653 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001653 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001653 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.29611 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.49551 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
2.89133 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.00124626 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
5 Yeast extract 1.99402 G_PER_L
6 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000498504 G_PER_L CHEBI:8806
Resazurin
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.199402 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0747757 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 Cellobiose
C12H22O11 342.297 g/mol
5.98205 G_PER_L CHEBI:17057
cellobiose
10 Cellulose
(C6H10O5)n.H2O 180.156 g/mol
9.97009 G_PER_L CHEBI:18246
(1->4)-beta-D-glucan
 Optional ingredient (MN 301)
11 Na2CO3
CO3.2Na 105.988 g/mol
1.49551 G_PER_L CHEBI:29377
sodium carbonate
12 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.498504 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
13 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
14 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve ingredients except magnesium chloride, calcium chloride, cellobiose, cysteine and carbonate, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add magnesium chloride, calcium chloride and cellobiose from anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Cellobiose has to be sterilized by filtration. Prior to inoculation add cysteine from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH to 7.2.
  2. Note: Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose powder MN 301 (MACHEREY-NAGEL).
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.511727Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 520
2026-03-13T06:19:06.215318Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001653
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.388940+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM" to "ruminoclostridium_cellulolyticum_cm3_medium" for consistent matching
2026-06-05T07:41:44.850221+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.149844+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.