moorella_thermoacetica_g_20_medium

Overview

External ontology

mediadive.medium:527 (source link unavailable) — MOORELLA THERMOACETICA (G-20) MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'clostridium_thermoaceticum_ii_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:527', 'original_category': 'bacterial'}

pH

6.9

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium527.pdf

Composition

flowchart LR
    CultureMech_001658["moorella_thermoacetica_g_20_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001658 --> ing0_Yeast_extract
    ing1_Tryptone_peptone["Tryptone peptone"]
    CultureMech_001658 --> ing1_Tryptone_peptone
    ing2_Na_pyruvate["Na-pyruvate"]
    CultureMech_001658 --> ing2_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing2_Na_pyruvate -.-> CHEBI_50144
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001658 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001658 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_001658 --> ing5_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing5_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing6_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001658 --> ing6_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing6_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing7_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001658 --> ing7_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing7_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001658 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_ZnCl2["ZnCl2"]
    CultureMech_001658 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001658 --> ing10_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing10_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001658 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001658 --> ing12_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Sodium_resazurin -.-> CHEBI_8806
    ing13_NaHCO3["NaHCO3"]
    CultureMech_001658 --> ing13_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing13_NaHCO3 -.-> CHEBI_32139
    ing14_K2HPO4["K2HPO4"]
    CultureMech_001658 --> ing14_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing14_K2HPO4 -.-> CHEBI_131527
    ing15_KH2PO4["KH2PO4"]
    CultureMech_001658 --> ing15_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing15_KH2PO4 -.-> CHEBI_63036
    ing16_D_Glucose["D-Glucose"]
    CultureMech_001658 --> ing16_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing16_D_Glucose -.-> CHEBI_17634
    ing17_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001658 --> ing17_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing17_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing18_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001658 --> ing18_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing18_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (19)

Preparation

1. Sparge solution A with 100% CO2 gas for 30 - 45 min to make it anoxic, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved under 80% N2 and 20% CO2 gas mixture. Solutions C, D and E are autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 6.9, if necessary

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• clostridium_thermoaceticum_ii_medium (CultureMech:005890)
• moorella_thermoacetica_g_20_medium (CultureMech:001658)