clostridium_thermoaceticum_ii_medium

CultureMech:005890 bacterial COMPLEX LIQUID
Original name: CLOSTRIDIUM THERMOACETICUM II medium

Overview

External ontology
komodo.medium:527 (source link unavailable) — CLOSTRIDIUM THERMOACETICUM II medium
Applications
  • Microbial cultivation
pH
6.9
Notes
Source: KOMODO ModelSEED | ID: 527 | DSMZ Medium: 527 (mediadive.medium:527) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_005890["clostridium_thermoaceticum_ii_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_005890 --> ing0_Yeast_extract
    ing1_Tryptone_peptone["Tryptone peptone"]
    CultureMech_005890 --> ing1_Tryptone_peptone
    ing2_Na_pyruvate["Na-pyruvate"]
    CultureMech_005890 --> ing2_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing2_Na_pyruvate -.-> CHEBI_50144
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005890 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005890 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_005890 --> ing5_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing5_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing6_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_005890 --> ing6_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing6_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing7_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_005890 --> ing7_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing7_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005890 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_ZnCl2["ZnCl2"]
    CultureMech_005890 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_005890 --> ing10_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing10_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005890 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Sodium_resazurin["Sodium resazurin"]
    CultureMech_005890 --> ing12_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Sodium_resazurin -.-> CHEBI_8806
    ing13_NaHCO3["NaHCO3"]
    CultureMech_005890 --> ing13_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing13_NaHCO3 -.-> CHEBI_32139
    ing14_K2HPO4["K2HPO4"]
    CultureMech_005890 --> ing14_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing14_K2HPO4 -.-> CHEBI_131527
    ing15_KH2PO4["KH2PO4"]
    CultureMech_005890 --> ing15_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing15_KH2PO4 -.-> CHEBI_63036
    ing16_D_Glucose["D-Glucose"]
    CultureMech_005890 --> ing16_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing16_D_Glucose -.-> CHEBI_17634
    ing17_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_005890 --> ing17_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing17_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing18_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_005890 --> ing18_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing18_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 Yeast extract 8.22368 G_PER_L
2 Tryptone peptone 8.22368 G_PER_L
3 Na-pyruvate
C3H3O3.Na 110.044 g/mol
2.96053 G_PER_L CHEBI:50144
sodium pyruvate
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.64474 G_PER_L CHEBI:62946
ammonium sulfate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.411184 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.0657895 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
7 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0493421 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
8 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.00542763 G_PER_L CHEBI:63939
sodium tungstate dihydrate
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.00394737 G_PER_L CHEBI:75213
sodium molybdate dihydrate
10 ZnCl2
2Cl.Zn 136.296 g/mol
0.00230263 G_PER_L CHEBI:49976
zinc dichloride
11 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.000493421 G_PER_L CHEBI:131361
disodium selenite pentahydrate
12 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.000328947 G_PER_L CHEBI:34887
nickel dichloride
13 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000822368 G_PER_L CHEBI:8806
Resazurin
14 NaHCO3
CHO3.Na 84.006 g/mol
56 G_PER_L CHEBI:32139
sodium hydrogencarbonate
15 K2HPO4
HO4P.2K 174.174 g/mol
23.3333 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
16 KH2PO4
H2O4P.K 136.084 g/mol
18.3333 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
17 D-Glucose
C6H12O6 180.156 g/mol
200 G_PER_L CHEBI:17634
D-glucose
18 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
50 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
19 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
50 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 527
2026-02-03T11:19:10.262735 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 527
2026-03-13T06:19:37.114464Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:005890
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:53.817580+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CLOSTRIDIUM THERMOACETICUM II medium" to "clostridium_thermoaceticum_ii_medium" for consistent matching
2026-06-05T07:40:54.292937+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.836127+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.