Overview
- External ontology
- mediadive.medium:574 (source link unavailable) — GUYPARKERIA HYDROTHERMALIS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 't_asw_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:574', 'original_category': 'bacterial'}
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium574.pdf
Composition
flowchart LR
CultureMech_001702["guyparkeria_hydrothermalis_medium"]
ing0_NaCl["NaCl"]
CultureMech_001702 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001702 --> ing1_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing1_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_001702 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001702 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_KH2PO4["KH2PO4"]
CultureMech_001702 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NaHCO3["NaHCO3"]
CultureMech_001702 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001702 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Phenol_red["Phenol red"]
CultureMech_001702 --> ing7_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing7_Phenol_red -.-> CHEBI_31991
ing8_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_001702 --> ing8_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing8_Tris_HCl_buffer -.-> CHEBI_9754
ing9_Na2_EDTA["Na2-EDTA"]
CultureMech_001702 --> ing9_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing9_Na2_EDTA -.-> CHEBI_64734
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001702 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001702 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001702 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_001702 --> ing13__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing13__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001702 --> ing14_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001702 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
25 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
5.8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.3, 5.5] |
| 8 |
Phenol red
C19H14O5S 354.383 g/mol
|
0.01 G_PER_L |
CHEBI:31991
phenol red
|
|
| 9 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
200 G_PER_L |
CHEBI:9754
tris
|
(0.1 M, pH 7.5) |
| 10 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
50 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
2.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
5.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
1.1 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 15 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.6 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.6 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Dissove ingredients and adjust pH to 7.5. Sterilize the medium by filtration.
- First dissolve EDTA in distilled water and adjust pH to 7 using 2 N KOH. Adjust pH of final solution to 6.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- guyparkeria_hydrothermalis_medium (CultureMech:001702)
- t_asw_medium (CultureMech:006021)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.630752Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 574 |
| 2026-03-13T03:09:33.640497Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:06.557342Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001702 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.764457+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GUYPARKERIA HYDROTHERMALIS MEDIUM" to "guyparkeria_hydrothermalis_medium" for consistent matching |
| 2026-06-05T07:41:06.797110+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.210635+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:36.575719+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: guyparkeria_hydrothermalis_medium, t_asw_medium |
| 2026-08-06T03:05:36.575754+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 0a3232a75abd124b82e22e2e41741a9a9356d58c715312c60e4af62b87651adc |