guyparkeria_hydrothermalis_medium

CultureMech:001702 bacterial DEFINED LIQUID
Original name: GUYPARKERIA HYDROTHERMALIS MEDIUM

Overview

External ontology
mediadive.medium:574 (source link unavailable) — GUYPARKERIA HYDROTHERMALIS MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium574.pdf

Composition

flowchart LR
    CultureMech_001702["guyparkeria_hydrothermalis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001702 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001702 --> ing1_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing1_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001702 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001702 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KH2PO4["KH2PO4"]
    CultureMech_001702 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NaHCO3["NaHCO3"]
    CultureMech_001702 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001702 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Phenol_red["Phenol red"]
    CultureMech_001702 --> ing7_Phenol_red
    CHEBI_31991["CHEBI:31991"]
    ing7_Phenol_red -.-> CHEBI_31991
    ing8_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_001702 --> ing8_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing8_Tris_HCl_buffer -.-> CHEBI_9754
    ing9_Na2_EDTA["Na2-EDTA"]
    CultureMech_001702 --> ing9_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na2_EDTA -.-> CHEBI_64734
    ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001702 --> ing10__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
2 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2.5 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 KH2PO4
H2O4P.K 136.084 g/mol
0.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 NaHCO3
CHO3.Na 84.006 g/mol
0.2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
5.8 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.3, 5.5]
8 Phenol red
C19H14O5S 354.383 g/mol
0.01 G_PER_L CHEBI:31991
phenol red
9 Tris-HCl buffer
C4H11NO3 121.136 g/mol
200 G_PER_L CHEBI:9754
tris
 (0.1 M, pH 7.5)
10 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
50 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
11 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
1.1 G_PER_L CHEBI:91249
ammonium molybdate

Preparation

  1. Dissove ingredients and adjust pH to 7.5. Sterilize the medium by filtration.
  2. First dissolve EDTA in distilled water and adjust pH to 7 using 2 N KOH. Adjust pH of final solution to 6.0 with KOH.

Solutions

Trace element solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 2.2 G_PER_L
MnCl2 x 4 H2O 5.1 G_PER_L
FeSO4 x 7 H2O 5 G_PER_L
CuSO4 x 5 H2O 1.6 G_PER_L
CoCl2 x 6 H2O 1.6 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.630752Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 574
2026-03-13T03:09:33.640497Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:06.557342Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001702
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:18.764457+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GUYPARKERIA HYDROTHERMALIS MEDIUM" to "guyparkeria_hydrothermalis_medium" for consistent matching
2026-06-05T07:41:06.797110+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.210635+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.285915+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 1 solution(s): 5 -> 'Trace element solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).