pyrobaculum_bs_medium

CultureMech:001740 archaea COMPLEX LIQUID
Original name: PYROBACULUM (BS) MEDIUM

Overview

External ontology
mediadive.medium:611 (source link unavailable) — PYROBACULUM (BS) MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium611.pdf

Composition

flowchart LR
    CultureMech_001740["pyrobaculum_bs_medium"]
    ing0_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_001740 --> ing0_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing0_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001740 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001740 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_KNO3["KNO3"]
    CultureMech_001740 --> ing3_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing3_KNO3 -.-> CHEBI_63043
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001740 --> ing4_Yeast_extract
    ing5_NaHCO3["NaHCO3"]
    CultureMech_001740 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_NaCl["NaCl"]
    CultureMech_001740 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001740 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001740 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001740 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10_Na2SO4["Na2SO4"]
    CultureMech_001740 --> ing10_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing10_Na2SO4 -.-> CHEBI_32149
    ing11_KCl["KCl"]
    CultureMech_001740 --> ing11_KCl
    CHEBI_32588["CHEBI:32588"]
    ing11_KCl -.-> CHEBI_32588
    ing12_Na2CO3["Na2CO3"]
    CultureMech_001740 --> ing12_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing12_Na2CO3 -.-> CHEBI_29377
    ing13_NaBr["NaBr"]
    CultureMech_001740 --> ing13_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing13_NaBr -.-> CHEBI_63004
    ing14_KBr["KBr"]
    CultureMech_001740 --> ing14_KBr
    CHEBI_32030["CHEBI:32030"]
    ing14_KBr -.-> CHEBI_32030
    ing15_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001740 --> ing15_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing15_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing16_H3BO3["H3BO3"]
    CultureMech_001740 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_Na2HPO4["Na2HPO4"]
    CultureMech_001740 --> ing17_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing17_Na2HPO4 -.-> CHEBI_34683
    ing18_NaF["NaF"]
    CultureMech_001740 --> ing18_NaF
    CHEBI_28741["CHEBI:28741"]
    ing18_NaF -.-> CHEBI_28741
    ing19_Na_silicate["Na-silicate"]
    CultureMech_001740 --> ing19_Na_silicate
    CHEBI_60720["CHEBI:60720"]
    ing19_Na_silicate -.-> CHEBI_60720
    ing20_KI["KI"]
    CultureMech_001740 --> ing20_KI
    CHEBI_8346["CHEBI:8346"]
    ing20_KI -.-> CHEBI_8346
    ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001740 --> ing21_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing22_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001740 --> ing22_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing22_MnSO4_x_H2O -.-> CHEBI_86364
    ing23_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001740 --> ing23_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing23_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing24_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001740 --> ing24_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing24_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing25_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001740 --> ing25_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing25_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing26_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001740 --> ing26_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing26_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing27_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001740 --> ing27_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing27_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001740 --> ing28_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001740 --> ing29_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
    CultureMech_001740 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.000197628 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.247036 G_PER_L CHEBI:31206
ammonium chloride
3 KH2PO4
H2O4P.K 136.084 g/mol
0.06917 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 KNO3
K.NO3 101.102 g/mol
0.988142 G_PER_L CHEBI:63043
potassium nitrate
5 Yeast extract 0.494071 G_PER_L
6 NaHCO3
CHO3.Na 84.006 g/mol
1.97628 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 NaCl
Cl.Na 58.443 g/mol
48.15 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 47.15, 1.0]
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
18.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
10.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 7.0, 3.0]
10 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.23 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 3.13, 0.1]
11 Na2SO4
2Na.O4S 142.043 g/mol
3.24 G_PER_L CHEBI:32149
sodium sulfate
12 KCl
Cl.K 74.551 g/mol
1.2 G_PER_L CHEBI:32588
potassium chloride
13 Na2CO3
CO3.2Na 105.988 g/mol
0.1 G_PER_L CHEBI:29377
sodium carbonate
14 NaBr
Br.Na 102.894 g/mol
0.1 G_PER_L CHEBI:63004
sodium bromide
15 KBr
Br.K 119.002 g/mol
0.08 G_PER_L CHEBI:32030
potassium bromide
16 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.072 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.062 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.052, 0.01]
18 Na2HPO4
HO4P.2Na 141.958 g/mol
0.0081 G_PER_L CHEBI:34683
disodium hydrogenphosphate
19 NaF
F.Na 41.988 g/mol
0.0024 G_PER_L CHEBI:28741
sodium fluoride
20 Na-silicate
2Na.O3Si 122.063 g/mol
0.0004 G_PER_L CHEBI:60720
sodium silicate
21 KI
I.K 166.002 g/mol
5e-05 G_PER_L CHEBI:8346
potassium iodide
22 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
23 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
24 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
25 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
26 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
27 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
28 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
29 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
30 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
31 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
32 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients (except nitrate, yeast extract and bicarbonate) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.0 and dispense under 100% N2 gas atmosphere into serum vials (e.g., 20 ml medium per 100 ml bottle). Replace gas phase with 80% H2 and 20% CO2 gas mixture and autoclave. Prior to inoculation add nitrate and yeast extract from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0 - 7.2.
  2. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:11.726384Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 611
2026-01-28T19:48:11.184664Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:08:45.994265Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:06.866551Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001740
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.096410+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYROBACULUM (BS) MEDIUM" to "pyrobaculum_bs_medium" for consistent matching
2026-05-20T06:02:03.404893+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:41:43.215262+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.768893+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:10.634012+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 10 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:32.349171+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 76137706370bada48457dd0dbc35209524af9955b367f587321eba9b10d6fc4a