Overview
- External ontology
- mediadive.medium:611 (source link unavailable) — PYROBACULUM (BS) MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium611.pdf
Composition
flowchart LR
CultureMech_001740["pyrobaculum_bs_medium"]
ing0_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_001740 --> ing0_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing0_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing1_NH4Cl["NH4Cl"]
CultureMech_001740 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KH2PO4["KH2PO4"]
CultureMech_001740 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_KNO3["KNO3"]
CultureMech_001740 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_Yeast_extract["Yeast extract"]
CultureMech_001740 --> ing4_Yeast_extract
ing5_NaHCO3["NaHCO3"]
CultureMech_001740 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_NaCl["NaCl"]
CultureMech_001740 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001740 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001740 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001740 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_Na2SO4["Na2SO4"]
CultureMech_001740 --> ing10_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing10_Na2SO4 -.-> CHEBI_32149
ing11_KCl["KCl"]
CultureMech_001740 --> ing11_KCl
CHEBI_32588["CHEBI:32588"]
ing11_KCl -.-> CHEBI_32588
ing12_Na2CO3["Na2CO3"]
CultureMech_001740 --> ing12_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing12_Na2CO3 -.-> CHEBI_29377
ing13_NaBr["NaBr"]
CultureMech_001740 --> ing13_NaBr
CHEBI_63004["CHEBI:63004"]
ing13_NaBr -.-> CHEBI_63004
ing14_KBr["KBr"]
CultureMech_001740 --> ing14_KBr
CHEBI_32030["CHEBI:32030"]
ing14_KBr -.-> CHEBI_32030
ing15_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001740 --> ing15_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing15_SrCl2_x_6_H2O -.-> CHEBI_36385
ing16_H3BO3["H3BO3"]
CultureMech_001740 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_Na2HPO4["Na2HPO4"]
CultureMech_001740 --> ing17_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing17_Na2HPO4 -.-> CHEBI_34683
ing18_NaF["NaF"]
CultureMech_001740 --> ing18_NaF
CHEBI_28741["CHEBI:28741"]
ing18_NaF -.-> CHEBI_28741
ing19_Na_silicate["Na-silicate"]
CultureMech_001740 --> ing19_Na_silicate
CHEBI_60720["CHEBI:60720"]
ing19_Na_silicate -.-> CHEBI_60720
ing20_KI["KI"]
CultureMech_001740 --> ing20_KI
CHEBI_8346["CHEBI:8346"]
ing20_KI -.-> CHEBI_8346
ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001740 --> ing21_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
ing22_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001740 --> ing22_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing22_MnSO4_x_H2O -.-> CHEBI_86364
ing23_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001740 --> ing23_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing23_FeSO4_x_7_H2O -.-> CHEBI_75836
ing24_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001740 --> ing24_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing24_CoSO4_x_7_H2O -.-> CHEBI_91244
ing25_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001740 --> ing25_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing26_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001740 --> ing26_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing26_CuSO4_x_5_H2O -.-> CHEBI_31440
ing27_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001740 --> ing27_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing27_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001740 --> ing28_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001740 --> ing29_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
CultureMech_001740 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.000197628 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.247036 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.06917 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
KNO3
K.NO3 101.102 g/mol
|
0.988142 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 5 | Yeast extract | 0.494071 G_PER_L | ||
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.97628 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
48.15 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 47.15, 1.0] |
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
18.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 7.0, 3.0] |
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.23 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 3.13, 0.1] |
| 11 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.24 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 12 |
KCl
Cl.K 74.551 g/mol
|
1.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 13 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.1 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 14 |
NaBr
Br.Na 102.894 g/mol
|
0.1 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 15 |
KBr
Br.K 119.002 g/mol
|
0.08 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 16 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.072 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.062 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.052, 0.01] |
| 18 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.0081 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 19 |
NaF
F.Na 41.988 g/mol
|
0.0024 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 20 |
Na-silicate
2Na.O3Si 122.063 g/mol
|
0.0004 G_PER_L |
CHEBI:60720
sodium silicate
|
|
| 21 |
KI
I.K 166.002 g/mol
|
5e-05 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 22 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 23 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 24 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 25 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 26 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 27 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 28 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 29 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 30 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 31 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 32 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients (except nitrate, yeast extract and bicarbonate) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.0 and dispense under 100% N2 gas atmosphere into serum vials (e.g., 20 ml medium per 100 ml bottle). Replace gas phase with 80% H2 and 20% CO2 gas mixture and autoclave. Prior to inoculation add nitrate and yeast extract from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0 - 7.2.
- After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.726384Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 611 |
| 2026-01-28T19:48:11.184664Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:45.994265Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:06.866551Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001740 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.096410+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYROBACULUM (BS) MEDIUM" to "pyrobaculum_bs_medium" for consistent matching |
| 2026-05-20T06:02:03.404893+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:41:43.215262+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.768893+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:10.634012+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 10 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |