Overview
- External ontology
- mediadive.medium:621 (source link unavailable) — PYGV AGAR
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'ekho_lake_strains_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a', 'original_category': 'bacterial'}
- {'name': 'medium_621_modified_for_dsm_45328', 'source': 'KOMODO', 'source_id': 'komodo.medium:621_45328', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11439', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11439', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11440', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11440', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11443', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11443', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11445', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11445', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11457', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11457', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11458', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11458', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11463', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11463', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11465', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11465', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11466', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11466', 'original_category': 'bacterial'}
- {'name': 'medium_621a_modified_for_dsm_11469', 'source': 'KOMODO', 'source_id': 'komodo.medium:621a_11469', 'original_category': 'bacterial'}
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium621.pdf
Composition
flowchart LR
CultureMech_001752["pygv_agar"]
ing0_Peptone["Peptone"]
CultureMech_001752 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001752 --> ing1_Yeast_extract
ing2_Agar["Agar"]
CultureMech_001752 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Glucose["Glucose"]
CultureMech_001752 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001752 --> ing4_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001752 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001752 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001752 --> ing7_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001752 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_Biotin["Biotin"]
CultureMech_001752 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Folic_acid["Folic acid"]
CultureMech_001752 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001752 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Riboflavine["Riboflavine"]
CultureMech_001752 --> ing12_Riboflavine
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavine -.-> CHEBI_17015
ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001752 --> ing13_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing14_Nicotinamide["Nicotinamide"]
CultureMech_001752 --> ing14_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing14_Nicotinamide -.-> CHEBI_17154
ing15_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_001752 --> ing15_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_D_Calcium_pantothenate -.-> CHEBI_31345
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_001752 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001752 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.25 G_PER_L | ||
| 2 | Yeast extract | 0.25 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10.5263 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
31.2632 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.51579 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0133368 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.104211 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.004 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.02 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Riboflavine
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 14 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 15 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.01 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 16 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 | Vitamin B12 | 0.0002 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Preparation
- Sterilize 20 min./121°C. After cooling to 60°C add to the medium:
- Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).
- Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.
- Store in the dark and cold (5°C).
Solutions
Metall salt sol. 44
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 13 source records.
- KOMODO_621a_EKHO_LAKE_STRAINS_medium (CultureMech:006128)
- medium_621_modified_for_dsm_45328 (CultureMech:006116)
- medium_621a_modified_for_dsm_11439 (CultureMech:006118)
- medium_621a_modified_for_dsm_11440 (CultureMech:006119)
- medium_621a_modified_for_dsm_11443 (CultureMech:006120)
- medium_621a_modified_for_dsm_11445 (CultureMech:006121)
- medium_621a_modified_for_dsm_11457 (CultureMech:006122)
- medium_621a_modified_for_dsm_11458 (CultureMech:006123)
- medium_621a_modified_for_dsm_11463 (CultureMech:006124)
- medium_621a_modified_for_dsm_11465 (CultureMech:006125)
- medium_621a_modified_for_dsm_11466 (CultureMech:006126)
- medium_621a_modified_for_dsm_11469 (CultureMech:006127)
- pygv_agar (CultureMech:001752)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.755792Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 621 |
| 2026-01-28T19:48:38.616484Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:04.657264+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:06.957060Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001752 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.198837+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYGV AGAR" to "pygv_agar" for consistent matching |
| 2026-05-16T09:04:29.865203+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:43.143818+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:56.366244+00:00 | recipe-merger | Merged 13 duplicate recipes into canonical record | Sources: KOMODO_621a_EKHO_LAKE_STRAINS_medium, medium_621_modified_for_dsm_45328, medium_621a_modified_for_dsm_11439, medium_621a_modified_for_dsm_11440, medium_621a_modified_for_dsm_11443, medium_621a_modified_for_dsm_11445, medium_621a_modified_for_dsm_11457, medium_621a_modified_for_dsm_11458, medium_621a_modified_for_dsm_11463, medium_621a_modified_for_dsm_11465, medium_621a_modified_for_dsm_11466, medium_621a_modified_for_dsm_11469, pygv_agar |
| 2026-08-06T03:04:56.366391+00:00 | merge_recipes.py | MERGED_RECIPES | merged 13 source recipe(s) on fingerprint a13271f0ac47dc5fe0513c09c64e308a8b4dd403750b78c473de7fdba2224206 |