medium_621a_modified_for_dsm_11469

CultureMech:006127 bacterial COMPLEX SOLID_AGAR
Original name: MEDIUM 621a MODIFIED FOR DSM 11469

Overview

External ontology
komodo.medium:621a_11469 (source link unavailable) — MEDIUM 621a MODIFIED FOR DSM 11469
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: KOMODO ModelSEED | ID: 621a_11469 | DSMZ Medium: 621a (mediadive.medium:621a) | Aerobic: No

Composition

flowchart LR
    CultureMech_006127["medium_621a_modified_for_dsm_11469"]
    ing0_Peptone["Peptone"]
    CultureMech_006127 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_006127 --> ing1_Yeast_extract
    ing2_Agar["Agar"]
    CultureMech_006127 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Glucose["Glucose"]
    CultureMech_006127 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_006127 --> ing4_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006127 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006127 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006127 --> ing7_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_006127 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Biotin["Biotin"]
    CultureMech_006127 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Folic_acid["Folic acid"]
    CultureMech_006127 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_006127 --> ing11_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing12_Riboflavine["Riboflavine"]
    CultureMech_006127 --> ing12_Riboflavine
    CHEBI_17015["CHEBI:17015"]
    ing12_Riboflavine -.-> CHEBI_17015
    ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_006127 --> ing13_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing14_Nicotinamide["Nicotinamide"]
    CultureMech_006127 --> ing14_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing14_Nicotinamide -.-> CHEBI_17154
    ing15_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_006127 --> ing15_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_D_Calcium_pantothenate -.-> CHEBI_31345
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_006127 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_006127 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Peptone 0.25 G_PER_L
2 Yeast extract 0.25 G_PER_L
3 Agar 15 G_PER_L CHEBI:2509
agar
4 Glucose
C6H12O6 180.156 g/mol
0.25 G_PER_L CHEBI:17234
glucose
5 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10.5263 G_PER_L CHEBI:44557
nitrilotriacetic acid
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
31.2632 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.51579 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0133368 G_PER_L CHEBI:75213
sodium molybdate dihydrate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.104211 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
11 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
12 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
13 Riboflavine
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
14 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
15 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
16 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
17 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
18 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid

Solutions

Metall salt sol. 44

Dose: 50 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 621a_11469
2026-02-03T11:19:08.161665 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 621
2026-03-09T08:04:43.547012+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:38.487940Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006127
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:55.418318+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM 621a MODIFIED FOR DSM 11469" to "medium_621a_modified_for_dsm_11469" for consistent matching
2026-05-16T09:04:18.036381+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:26.067230+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.