modified_thermus_162_medium (CultureMech:001761)

Overview

External ontology

mediadive.medium:630 (source link unavailable) — MODIFIED THERMUS 162 MEDIUM

Applications

Microbial cultivation

Synonyms

{'name': 'medium_630_modified_for_dsm_12048', 'source': 'KOMODO', 'source_id': 'komodo.medium:630_12048', 'original_category': 'bacterial'}
{'name': 'medium_630_modified_for_dsm_12049', 'source': 'KOMODO', 'source_id': 'komodo.medium:630_12049', 'original_category': 'bacterial'}
{'name': 'medium_630_modified_for_dsm_22673', 'source': 'KOMODO', 'source_id': 'komodo.medium:630_22673', 'original_category': 'bacterial'}
{'name': 'medium_630_modified_for_dsm_4252', 'source': 'KOMODO', 'source_id': 'komodo.medium:630_4252', 'original_category': 'bacterial'}
{'name': 'medium_630_modified_for_dsm_4253', 'source': 'KOMODO', 'source_id': 'komodo.medium:630_4253', 'original_category': 'bacterial'}

pH

7.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium630.pdf

Composition

flowchart LR
    CultureMech_001761["modified_thermus_162_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001761 --> ing0_Yeast_extract
    ing1_Tryptone["Tryptone"]
    CultureMech_001761 --> ing1_Tryptone
    ing2_Agar["Agar"]
    CultureMech_001761 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001761 --> ing3_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing3_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_001761 --> ing4_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing4_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001761 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_001761 --> ing6_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing6_Fe_III__citrate -.-> CHEBI_144421
    ing7_KH2PO4["KH2PO4"]
    CultureMech_001761 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_001761 --> ing8_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing8_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001761 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001761 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_CoCl2_x_4_H2O["CoCl2 x 4 H2O"]
    CultureMech_001761 --> ing11_CoCl2_x_4_H2O
    CHEBI_35696["CHEBI:35696"]
    ing11_CoCl2_x_4_H2O -.-> CHEBI_35696
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001761 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001761 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_H3BO3["H3BO3"]
    CultureMech_001761 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001761 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887

Ingredients (16)

#	Ingredient	Concentration	Ontology	Notes
1	Yeast extract	2.5 G_PER_L
2	Tryptone	2.5 G_PER_L
3	Agar	28 G_PER_L	CHEBI:2509 agar
4	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	12.9 G_PER_L	CHEBI:44557 nitrilotriacetic acid	[Merged 2 duplicates: 0.1, 12.8]
5	CaSO4 x 2 H2O Ca.2H2O.O4S 172.171 g/mol	0.04 G_PER_L	CHEBI:32583 calcium sulfate dihydrate
6	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.2 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
7	Fe(III) citrate C6H5FeO7 244.944 g/mol	0.00122472 G_PER_L	CHEBI:144421 iron(III) citrate
8	KH2PO4 H2O4P.K 136.084 g/mol	5.44 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
9	Na2HPO4 x 12 H2O HO4P.2Na 141.958 g/mol	43 G_PER_L	CHEBI:34683 disodium hydrogenphosphate
10	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	1 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate
11	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.5 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate
12	CoCl2 x 4 H2O 2Cl.Co 129.839 g/mol	0.3 G_PER_L	CHEBI:35696 cobalt dichloride
13	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.05 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate
14	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.05 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
15	H3BO3 H3BO3 61.833 g/mol	0.02 G_PER_L	CHEBI:33118 boric acid
16	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.02 G_PER_L	CHEBI:34887 nickel dichloride

Preparation

Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min. Autoclave the phosphate buffer separately and then add to the medium.
Adjust pH to 7.2
Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 8 - 9 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

Target organisms

No target organisms are recorded.

Provenance

Merged from 6 source records.

medium_630_modified_for_dsm_12048 (CultureMech:006140)
medium_630_modified_for_dsm_12049 (CultureMech:006141)
medium_630_modified_for_dsm_22673 (CultureMech:006142)
medium_630_modified_for_dsm_4252 (CultureMech:006143)
medium_630_modified_for_dsm_4253 (CultureMech:006144)
modified_thermus_162_medium (CultureMech:001761)

Timestamp	Curator	Action	Notes
2026-01-26T17:53:11.779841Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 630
2026-03-13T03:09:34.405849Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:19:07.027246Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001761
2026-03-17T06:30:45.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.281167+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MODIFIED THERMUS 162 MEDIUM" to "modified_thermus_162_medium" for consistent matching
2026-06-05T07:41:37.904037+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.999215+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:42.761384+00:00	recipe-merger	Merged 6 duplicate recipes into canonical record	Sources: medium_630_modified_for_dsm_12048, medium_630_modified_for_dsm_12049, medium_630_modified_for_dsm_22673, medium_630_modified_for_dsm_4252, medium_630_modified_for_dsm_4253, modified_thermus_162_medium
2026-08-06T03:05:42.761452+00:00	merge_recipes.py	MERGED_RECIPES	merged 6 source recipe(s) on fingerprint 4af53d83b7103baff61d460153c1b8a868945490dea98f8225eca5a49af43529