Overview
- External ontology
- mediadive.medium:634c (source link unavailable) — RCM MEDIUM (N2/CO2)
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'rcm_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:634', 'original_category': 'bacterial'}
- {'name': 'rcm_medium_halophilic_clostridia', 'source': 'KOMODO', 'source_id': 'komodo.medium:634b', 'original_category': 'bacterial'}
- {'name': 'rcm_medium_modified', 'source': 'KOMODO', 'source_id': 'komodo.medium:634a', 'original_category': 'bacterial'}
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium634c.pdf
Composition
flowchart LR
CultureMech_001768["rcm_medium_n2_co2"]
ing0_dehydrated_RCM_medium["dehydrated RCM medium"]
CultureMech_001768 --> ing0_dehydrated_RCM_medium
ing1_Sodium_resazurin["Sodium resazurin"]
CultureMech_001768 --> ing1_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Sodium_resazurin -.-> CHEBI_8806
ing2_Sodium_carbonate["Sodium carbonate"]
CultureMech_001768 --> ing2_Sodium_carbonate
CHEBI_29377["CHEBI:29377"]
ing2_Sodium_carbonate -.-> CHEBI_29377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | dehydrated RCM medium | 38 G_PER_L | ||
| 2 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 3 |
Sodium carbonate
CO3.2Na 105.988 g/mol
|
1 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- Dissolve dehydrated RCM medium (available from BD BBL, OXOID, or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v). Bring to a boil and cool to room temperature while sparging with an 80% N2 and 20% CO2 gas mixture. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 15 min at 121°C. Before use, add 1.0 g of sodium carbonate per liter medium from a sterile anoxic stock solution prepared under an 80% N2 and 20% CO2 gas mixture. Check the pH of the medium after autoclaving and adjust the pH to 6.8 if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 4 source records.
- KOMODO_634_RCM_medium (CultureMech:006148)
- rcm_medium_halophilic_clostridia (CultureMech:006150)
- rcm_medium_modified (CultureMech:006149)
- rcm_medium_n2_co2 (CultureMech:001768)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.791507Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 634c |
| 2026-01-28T19:48:36.071771Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:07.058702Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001768 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.317631+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RCM MEDIUM (N2/CO2)" to "rcm_medium_n2_co2" for consistent matching |
| 2026-06-05T07:41:43.913401+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:56.467111+00:00 | recipe-merger | Merged 4 duplicate recipes into canonical record | Sources: KOMODO_634_RCM_medium, rcm_medium_halophilic_clostridia, rcm_medium_modified, rcm_medium_n2_co2 |
| 2026-08-06T03:04:56.467160+00:00 | merge_recipes.py | MERGED_RECIPES | merged 4 source recipe(s) on fingerprint be022f10bb653cbcc33a85471b432aca2525c18484815e2211f18b18dd844d53 |