rcm_medium_n2_co2

Overview

External ontology

mediadive.medium:634c (source link unavailable) — RCM MEDIUM (N2/CO2)

Applications

• Microbial cultivation

pH

6.8

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium634c.pdf

Composition

flowchart LR
    CultureMech_001768["rcm_medium_n2_co2"]
    ing0_dehydrated_RCM_medium["dehydrated RCM medium"]
    CultureMech_001768 --> ing0_dehydrated_RCM_medium
    ing1_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001768 --> ing1_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing1_Sodium_resazurin -.-> CHEBI_8806
    ing2_Sodium_carbonate["Sodium carbonate"]
    CultureMech_001768 --> ing2_Sodium_carbonate
    CHEBI_29377["CHEBI:29377"]
    ing2_Sodium_carbonate -.-> CHEBI_29377

Ingredients (3)

Preparation

1. Dissolve dehydrated RCM medium (available from BD BBL, OXOID, or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v). Bring to a boil and cool to room temperature while sparging with an 80% N2 and 20% CO2 gas mixture. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 15 min at 121°C. Before use, add 1.0 g of sodium carbonate per liter medium from a sterile anoxic stock solution prepared under an 80% N2 and 20% CO2 gas mixture. Check the pH of the medium after autoclaving and adjust the pH to 6.8 if necessary.

Target organisms

No target organisms are recorded.

Provenance