desulfovibrio_medium_choline

CultureMech:001776 bacterial COMPLEX LIQUID
Original name: DESULFOVIBRIO MEDIUM (CHOLINE)

Overview

External ontology
mediadive.medium:63c (source link unavailable) — DESULFOVIBRIO MEDIUM (CHOLINE)
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63c.pdf

Composition

flowchart LR
    CultureMech_001776["desulfovibrio_medium_choline"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001776 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001776 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2SO4["Na2SO4"]
    CultureMech_001776 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001776 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001776 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Choline_chloride["Choline chloride"]
    CultureMech_001776 --> ing5_Choline_chloride
    CHEBI_133341["CHEBI:133341"]
    ing5_Choline_chloride -.-> CHEBI_133341
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001776 --> ing6_Yeast_extract
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001776 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001776 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Na_thioglycolate["Na-thioglycolate"]
    CultureMech_001776 --> ing9_Na_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing9_Na_thioglycolate -.-> CHEBI_86481
    ing10_Ascorbic_acid["Ascorbic acid"]
    CultureMech_001776 --> ing10_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing10_Ascorbic_acid -.-> CHEBI_22652

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.510204 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
1.02041 G_PER_L CHEBI:31206
ammonium chloride
3 Na2SO4
2Na.O4S 142.043 g/mol
1.02041 G_PER_L CHEBI:32149
sodium sulfate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.102041 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2.04082 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Choline chloride
C5H14NO.Cl 139.626 g/mol
5.10204 G_PER_L CHEBI:133341
choline chloride
7 Yeast extract 1.02041 G_PER_L
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
50 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
10 G_PER_L CHEBI:86481
sodium thioglycolate
11 Ascorbic acid 10 G_PER_L CHEBI:22652
ascorbic acid

Preparation

  1. Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Check medium pH after autoclaving and adjust to 6.8 - 7.0, if necessary.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:10.077224Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 63c
2026-03-13T06:19:07.113224Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001776
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.376969+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOVIBRIO MEDIUM (CHOLINE)" to "desulfovibrio_medium_choline" for consistent matching
2026-05-20T06:01:14.899817+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:40:57.898874+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.074516+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:32.263640+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint d557066ec6de29aaed6456e8a05aa93848f4b7d5d094a1596e7146f503484d81