desulfovibrio_medium_choline

CultureMech:001776 bacterial COMPLEX LIQUID
Original name: DESULFOVIBRIO MEDIUM (CHOLINE)

Overview

External ontology
mediadive.medium:63c (source link unavailable) — DESULFOVIBRIO MEDIUM (CHOLINE)
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
DSMZ Medium 63c prepares DESULFOVIBRIO MEDIUM (CHOLINE) as 980 ml Solution A plus 10 ml Solution B and 10 ml Solution C, then adjusts the combined medium to pH 7.8, distributes it under 100% N2, autoclaves it for 15 minutes at 121 C, and checks for a final pH of 6.8-7.0.

Composition

flowchart LR
    CultureMech_001776["desulfovibrio_medium_choline"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001776 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001776 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2SO4["Na2SO4"]
    CultureMech_001776 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001776 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001776 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Choline_chloride__SIGMA_["Choline chloride (SIGMA)"]
    CultureMech_001776 --> ing5_Choline_chloride__SIGMA_
    CHEBI_133341["CHEBI:133341"]
    ing5_Choline_chloride__SIGMA_ -.-> CHEBI_133341
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001776 --> ing6_Yeast_extract
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001776 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_Distilled_water["Distilled water"]
    CultureMech_001776 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001776 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_Na_thioglycolate["Na-thioglycolate"]
    CultureMech_001776 --> ing10_Na_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing10_Na_thioglycolate -.-> CHEBI_86481
    ing11_Ascorbic_acid["Ascorbic acid"]
    CultureMech_001776 --> ing11_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing11_Ascorbic_acid -.-> CHEBI_22652

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.50 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 DSMZ Medium 63c lists 0.50 g K2HPO4 in the final 1000 ml medium.
2 NH4Cl
Cl.H4N 53.492 g/mol
1.00 G_PER_L CHEBI:31206
ammonium chloride
 DSMZ Medium 63c lists 1.00 g NH4Cl in the final 1000 ml medium.
3 Na2SO4
2Na.O4S 142.043 g/mol
1.00 G_PER_L CHEBI:32149
sodium sulfate
 DSMZ Medium 63c lists 1.00 g Na2SO4 in the final 1000 ml medium.
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.10 G_PER_L CHEBI:86158
calcium chloride dihydrate
 DSMZ Medium 63c lists 0.10 g CaCl2 x 2 H2O in the final medium.
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2.00 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 DSMZ Medium 63c lists 2.00 g MgSO4 x 7 H2O in the final medium.
6 Choline chloride (SIGMA)
C5H14NO.Cl 139.626 g/mol
5.00 G_PER_L CHEBI:133341
choline chloride
 DSMZ Medium 63c lists 5.00 g Choline chloride (SIGMA) in the final medium.
7 Yeast extract 1.00 G_PER_L FOODON:03315426
yeast extract
 DSMZ Medium 63c lists 1.00 g yeast extract in the final medium.
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
 DSMZ Medium 63c adds 0.50 ml of 0.1% w/v sodium resazurin, equivalent to 0.0005 g/L in the final medium.
9 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 DSMZ Medium 63c combines 980 ml distilled water in Solution A, 10 ml in Solution B, and 10 ml in Solution C.
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.50 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 DSMZ Medium 63c lists 0.50 g FeSO4 x 7 H2O in Solution B.
11 Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
0.10 G_PER_L CHEBI:86481
sodium thioglycolate
 DSMZ Medium 63c lists 0.10 g Na-thioglycolate in Solution C.
12 Ascorbic acid 0.10 G_PER_L CHEBI:22652
ascorbic acid
 DSMZ Medium 63c lists 0.10 g ascorbic acid in Solution C.

Preparation

  1. Dissolve the Solution A ingredients in 980 ml distilled water.
  2. Bring Solution A to the boil, then cool to room temperature while sparging with 100% N2 gas.
  3. Add Solution B, containing FeSO4 x 7 H2O in 10 ml distilled water, and Solution C, containing Na-thioglycolate and ascorbic acid in 10 ml distilled water.
  4. Adjust pH to 7.8 with NaOH.
  5. Distribute under 100% N2 gas in anoxic Hungate-type tubes while continuously swirling the medium to keep the grey precipitate suspended.
  6. Autoclave for 15 minutes at 121 C.
  7. After autoclaving, check the medium pH and adjust to pH 6.8-7.0 if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.077224Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 63c
2026-03-13T06:19:07.113224Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001776
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.376969+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOVIBRIO MEDIUM (CHOLINE)" to "desulfovibrio_medium_choline" for consistent matching
2026-05-20T06:01:14.899817+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:40:57.898874+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.074516+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-08T00:00:00-07:00 repair_komodo_272_score35.py RESOLVED_KOMODO_272_SCORE35 DSMZ Medium 63c explicitly lists Solution A at 980 ml, Solution B at 10 ml, and Solution C at 10 ml, making the corrected FeSO4 x 7 H2O, Na-thioglycolate, and ascorbic acid amounts final-medium rather than stock-strength concentrations.
2026-09-08T00:00:00-07:00 repair_komodo_272_score35.py LINKED_KOMODO_272_SCORE35_CHILD Added or refreshed reciprocal parent-child link for data/normalized_yaml/bacterial/KOMODO_272_DESULFOVIBRIO_medium_choline.yaml.