Overview
- External ontology
- mediadive.medium:687 (source link unavailable) — COLUMBIA BLOOD AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium687.pdf
Composition
flowchart LR
CultureMech_001823["columbia_blood_agar"]
ing0_Defibrinated_Blood["Defibrinated Blood"]
CultureMech_001823 --> ing0_Defibrinated_Blood
ing1_Bacto_Panton["Bacto Panton"]
CultureMech_001823 --> ing1_Bacto_Panton
ing2_Bacto_Bitone["Bacto Bitone"]
CultureMech_001823 --> ing2_Bacto_Bitone
ing3_Tryptic_Digest_of_beef_heart["Tryptic Digest of beef heart"]
CultureMech_001823 --> ing3_Tryptic_Digest_of_beef_heart
ing4_Corn_starch["Corn starch"]
CultureMech_001823 --> ing4_Corn_starch
CHEBI_28017["CHEBI:28017"]
ing4_Corn_starch -.-> CHEBI_28017
ing5_NaCl["NaCl"]
CultureMech_001823 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Agar["Agar"]
CultureMech_001823 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Defibrinated Blood | 50 G_PER_L | ||
| 2 | Bacto Panton | 10 G_PER_L | ||
| 3 | Bacto Bitone | 10 G_PER_L | ||
| 4 | Tryptic Digest of beef heart | 3 G_PER_L | ||
| 5 | Corn starch | 1 G_PER_L |
CHEBI:28017
starch
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Suspend 44 g Columbia Blood Agar Base in 1l deionized water. Heat to boiling to dissolve completely.
- Sterilize in autoclave for 15 min at 121°C.
- Cool to 45°C. Add 5% sterile defibrinated blood and mix well.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- DSMZ_687_COLUMBIA_BLOOD_AGAR (CultureMech:001823)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.927805Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 687 |
| 2026-01-28T19:48:30.378147Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:07.490434Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001823 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.812289+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "COLUMBIA BLOOD AGAR" to "columbia_blood_agar" for consistent matching |
| 2026-06-05T07:40:16.265409+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:34.819875+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bdf37fcaa52fd4cf878f3be00061d43b857a4bd0895a051bd4527a2f73ce22c8 |