Overview
- External ontology
- mediadive.medium:687 (source link unavailable) — COLUMBIA BLOOD AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium687.pdf
Composition
flowchart LR
CultureMech_001823["columbia_blood_agar"]
ing0_Bacto_Panton__Peptone_["Bacto Panton (Peptone)"]
CultureMech_001823 --> ing0_Bacto_Panton__Peptone_
ing1_Bacto_Bitone__Peptone_["Bacto Bitone (Peptone)"]
CultureMech_001823 --> ing1_Bacto_Bitone__Peptone_
ing2_Tryptic_Digest_of_beef_heart["Tryptic Digest of beef heart"]
CultureMech_001823 --> ing2_Tryptic_Digest_of_beef_heart
ing3_Corn_starch["Corn starch"]
CultureMech_001823 --> ing3_Corn_starch
CHEBI_28017["CHEBI:28017"]
ing3_Corn_starch -.-> CHEBI_28017
ing4_NaCl["NaCl"]
CultureMech_001823 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Agar["Agar"]
CultureMech_001823 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Deionized_water["Deionized water"]
CultureMech_001823 --> ing6_Deionized_water
CHEBI_15377["CHEBI:15377"]
ing6_Deionized_water -.-> CHEBI_15377
ing7_Sterile_defibrinated_blood["Sterile defibrinated blood"]
CultureMech_001823 --> ing7_Sterile_defibrinated_blood
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Panton (Peptone) | 10 G_PER_L | DSMZ Medium 687 lists Bacto Panton as a peptone in the Columbia Blood Agar Base; the BD product is not reducible to one ChEBI molecule. | |
| 2 | Bacto Bitone (Peptone) | 10 G_PER_L | DSMZ Medium 687 lists Bacto Bitone as a peptone in the Columbia Blood Agar Base; the BD product is not reducible to one ChEBI molecule. | |
| 3 | Tryptic Digest of beef heart | 3 G_PER_L | DSMZ Medium 687 lists tryptic digest of beef heart as part of the Columbia Blood Agar Base. | |
| 4 | Corn starch | 1 G_PER_L |
CHEBI:28017
starch
|
DSMZ Medium 687 lists 1 g corn starch. |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
DSMZ Medium 687 lists 5 g NaCl. |
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
DSMZ Medium 687 lists 15 g agar. |
| 7 |
Deionized water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
DSMZ Medium 687 instructs suspending Columbia Blood Agar Base in 1 L deionized water. |
| 8 | Sterile defibrinated blood | 5 PERCENT_V_V |
UBERON:0000178
blood
|
DSMZ Medium 687 instructs adding 5% sterile defibrinated blood after cooling. |
Preparation
- Suspend 44 g Columbia Blood Agar Base in 1 L deionized water and heat to boiling.
- Sterilize in an autoclave for 15 min at 121 C.
- Cool to 45 C, add 5% sterile defibrinated blood, and mix well.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.927805Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 687 |
| 2026-01-28T19:48:30.378147Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:07.490434Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001823 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.812289+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "COLUMBIA BLOOD AGAR" to "columbia_blood_agar" for consistent matching |
| 2026-06-05T07:40:16.265409+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_dsmz_1551_687_score15.py | RESOLVED_DSMZ_1551_687_SCORE15 | DSMZ Medium 687 lists Columbia Blood Agar Base as Bacto Panton, Bacto Bitone, tryptic digest of beef heart, corn starch, NaCl, and agar suspended in 1 L deionized water; after sterilization and cooling it adds 5% sterile defibrinated blood. |