Overview
- External ontology
- mediadive.medium:692 (source link unavailable) — METHANOSALSUM WeN5 MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'alkaliphilic_methanogen_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:692', 'original_category': 'archaea'}
- pH range
- {'min': 9.2, 'max': 9.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium692.pdf
Composition
flowchart LR
CultureMech_001829["methanosalsum_wen5_medium"]
ing0_NaCl["NaCl"]
CultureMech_001829 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NaHCO3["NaHCO3"]
CultureMech_001829 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_KH2PO4["KH2PO4"]
CultureMech_001829 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_001829 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001829 --> ing4_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing4_NiCl2_x_6_H2O -.-> CHEBI_34887
ing5_Yeast_extract["Yeast extract"]
CultureMech_001829 --> ing5_Yeast_extract
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_001829 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_Na2CO3["Na2CO3"]
CultureMech_001829 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
ing8_Methanol["Methanol"]
CultureMech_001829 --> ing8_Methanol
CHEBI_17790["CHEBI:17790"]
ing8_Methanol -.-> CHEBI_17790
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001829 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001829 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001829 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001829 --> ing12_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing12_MnSO4_x_H2O -.-> CHEBI_86364
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001829 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001829 --> ing14_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001829 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001829 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001829 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001829 --> ing18_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing19_H3BO3["H3BO3"]
CultureMech_001829 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001829 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001829 --> ing21_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001829 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
15.6771 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 14.6771, 1.0] |
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
9.78474 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.293542 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.489237 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03195695 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.00195695, 0.03] |
| 6 | Yeast extract | 0.489237 G_PER_L | ||
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000489237 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
3.91389 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 9 |
Methanol
CH4O 32.042 g/mol
|
7.74951 G_PER_L |
CHEBI:17790
methanol
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.978474 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients except carbonate, methanol and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and adjust pH to 9.0 - 9.2, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add methanol (50% v/v solution) and sulfide from anoxic stock solutions autoclaved under 100% N2 gas. Adjust pH of complete medium to 9.2 - 9.4.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- alkaliphilic_methanogen_medium (CultureMech:006297)
- methanosalsum_wen5_medium (CultureMech:001829)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.945811Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 692 |
| 2026-03-13T03:08:39.777167Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:07.564864Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001829 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.891663+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOSALSUM WeN5 MEDIUM" to "methanosalsum_wen5_medium" for consistent matching |
| 2026-05-20T06:01:56.220415+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '9.2-9.4' -> {'min': 9.2, 'max': 9.4} |
| 2026-06-05T07:41:32.309645+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.267621+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.210593+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: alkaliphilic_methanogen_medium, methanosalsum_wen5_medium |
| 2026-08-06T03:04:28.210650+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ecc81eb9510fdc7dc22b307c658d33cd86c3c17a0884b5b2ead7cf8525bd214d |