methanosalsum_wen5_medium

CultureMech:001829 archaea COMPLEX LIQUID
Original name: METHANOSALSUM WeN5 MEDIUM

Overview

External ontology
mediadive.medium:692 (source link unavailable) — METHANOSALSUM WeN5 MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 9.2, 'max': 9.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium692.pdf

Composition

flowchart LR
    CultureMech_001829["methanosalsum_wen5_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001829 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NaHCO3["NaHCO3"]
    CultureMech_001829 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001829 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001829 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001829 --> ing4_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing4_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_001829 --> ing5_Yeast_extract
    ing6_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001829 --> ing6_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Sodium_resazurin -.-> CHEBI_8806
    ing7_Na2CO3["Na2CO3"]
    CultureMech_001829 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_Methanol["Methanol"]
    CultureMech_001829 --> ing8_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing8_Methanol -.-> CHEBI_17790
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001829 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001829 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001829 --> ing11_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing12_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001829 --> ing12_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing12_MnSO4_x_H2O -.-> CHEBI_86364
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001829 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001829 --> ing14_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001829 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001829 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001829 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001829 --> ing18_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing19_H3BO3["H3BO3"]
    CultureMech_001829 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001829 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001829 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001829 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
15.6771 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 14.6771, 1.0]
2 NaHCO3
CHO3.Na 84.006 g/mol
9.78474 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.293542 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.489237 G_PER_L CHEBI:31206
ammonium chloride
5 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03195695 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.00195695, 0.03]
6 Yeast extract 0.489237 G_PER_L
7 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000489237 G_PER_L CHEBI:8806
Resazurin
8 Na2CO3
CO3.2Na 105.988 g/mol
3.91389 G_PER_L CHEBI:29377
sodium carbonate
9 Methanol
CH4O 32.042 g/mol
7.74951 G_PER_L CHEBI:17790
methanol
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.978474 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
12 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
13 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients except carbonate, methanol and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and adjust pH to 9.0 - 9.2, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add methanol (50% v/v solution) and sulfide from anoxic stock solutions autoclaved under 100% N2 gas. Adjust pH of complete medium to 9.2 - 9.4.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.945811Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 692
2026-03-13T03:08:39.777167Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:07.564864Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001829
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.891663+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOSALSUM WeN5 MEDIUM" to "methanosalsum_wen5_medium" for consistent matching
2026-05-20T06:01:56.220415+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '9.2-9.4' -> {'min': 9.2, 'max': 9.4}
2026-06-05T07:41:32.309645+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.267621+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.