Overview
- External ontology
- mediadive.medium:69 (source link unavailable) — STARKEYA NOVELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium69.pdf
Composition
flowchart LR
CultureMech_001837["starkeya_novella_medium"]
ing0_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_001837 --> ing0_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing0_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing1_KH2PO4["KH2PO4"]
CultureMech_001837 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_001837 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Yeast_extract["Yeast extract"]
CultureMech_001837 --> ing3_Yeast_extract
ing4_Phenol_red["Phenol red"]
CultureMech_001837 --> ing4_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing4_Phenol_red -.-> CHEBI_31991
ing5_Agar["Agar"]
CultureMech_001837 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001837 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001837 --> ing7_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing8_Na2_EDTA["Na2-EDTA"]
CultureMech_001837 --> ing8_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing8_Na2_EDTA -.-> CHEBI_64734
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001837 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001837 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001837 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001837 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_001837 --> ing13__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing13__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001837 --> ing14_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001837 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
11.7517 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.66297 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.332594 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 | Yeast extract | 0.332594 G_PER_L | ||
| 5 |
Phenol red
C19H14O5S 354.383 g/mol
|
0.00221729 G_PER_L |
CHEBI:31991
phenol red
|
|
| 6 | Agar | 16.6297 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
100 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 9 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
50 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
5.54 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
5.06 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
1.1 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 15 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.57 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.61 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Solutions A, B, C and D are sterilized separately by autoclaving and mixed when cooled to room temperature. Adjust pH of complete medium to 8.5 with sterile 0.5 N NaOH.
- Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- starkeya_novella_medium (CultureMech:001837)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.084382Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 69 |
| 2026-03-13T06:19:07.604768Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001837 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.934723+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "STARKEYA NOVELLA MEDIUM" to "starkeya_novella_medium" for consistent matching |
| 2026-06-05T07:41:46.999223+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.838421+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.618688+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e0d627f398d2026a55e55dbf2e2e9db2dfa23ed10877102e5d83f0bb13f190c6 |