starkeya_novella_medium

CultureMech:001837 bacterial COMPLEX SOLID_AGAR
Original name: STARKEYA NOVELLA MEDIUM

Overview

External ontology
mediadive.medium:69 (source link unavailable) — STARKEYA NOVELLA MEDIUM
Applications
  • Microbial cultivation
pH
8.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium69.pdf

Composition

flowchart LR
    CultureMech_001837["starkeya_novella_medium"]
    ing0_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_001837 --> ing0_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001837 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001837 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001837 --> ing3_Yeast_extract
    ing4_Phenol_red["Phenol red"]
    CultureMech_001837 --> ing4_Phenol_red
    CHEBI_31991["CHEBI:31991"]
    ing4_Phenol_red -.-> CHEBI_31991
    ing5_Agar["Agar"]
    CultureMech_001837 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001837 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001837 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_Na2_EDTA["Na2-EDTA"]
    CultureMech_001837 --> ing8_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing8_Na2_EDTA -.-> CHEBI_64734
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001837 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001837 --> ing10__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
11.7517 G_PER_L CHEBI:34683
disodium hydrogenphosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.66297 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.332594 G_PER_L CHEBI:31206
ammonium chloride
4 Yeast extract 0.332594 G_PER_L
5 Phenol red
C19H14O5S 354.383 g/mol
0.00221729 G_PER_L CHEBI:31991
phenol red
6 Agar 16.6297 G_PER_L CHEBI:2509
agar
 Optional ingredient (for solid medium)
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
100 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
50 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
10 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
5.54 G_PER_L CHEBI:86158
calcium chloride dihydrate
11 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
1.1 G_PER_L CHEBI:91249
ammonium molybdate

Preparation

  1. Solutions A, B, C and D are sterilized separately by autoclaving and mixed when cooled to room temperature. Adjust pH of complete medium to 8.5 with sterile 0.5 N NaOH.
  2. Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Solutions

Trace element solution (Vishniac & Santer, 1957)

Dose: 5 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 22 G_PER_L
MnCl2 x 4 H2O 5.06 G_PER_L
FeSO4 x 7 H2O 5 G_PER_L
CuSO4 x 5 H2O 1.57 G_PER_L
CoCl2 x 6 H2O 1.61 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.084382Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 69
2026-03-13T06:19:07.604768Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001837
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.934723+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "STARKEYA NOVELLA MEDIUM" to "starkeya_novella_medium" for consistent matching
2026-06-05T07:41:46.999223+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.838421+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.991460+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 1 solution(s): 5 -> 'Trace element solution (Vishniac & Santer, 1957)' @ 5 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).