Overview
- External ontology
- mediadive.medium:705 (source link unavailable) — BACILLUS INFERNUS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'msa_fe_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:705', 'original_category': 'bacterial'}
- {'name': 'msa_fe_medium_replace_na_lactate_with_na_formate', 'source': 'KOMODO', 'source_id': 'komodo.medium:705_replace_Na-lactate_with_Na-formate', 'original_category': 'bacterial'}
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium705.pdf
Composition
flowchart LR
CultureMech_001844["bacillus_infernus_medium"]
ing0_Ferric_citrate["Ferric citrate"]
CultureMech_001844 --> ing0_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing0_Ferric_citrate -.-> CHEBI_144421
ing1_NaCl["NaCl"]
CultureMech_001844 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NH4Cl["NH4Cl"]
CultureMech_001844 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001844 --> ing3_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001844 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Yeast_extract["Yeast extract"]
CultureMech_001844 --> ing5_Yeast_extract
ing6_Trypticase_peptone["Trypticase peptone"]
CultureMech_001844 --> ing6_Trypticase_peptone
ing7_Na_acetate["Na-acetate"]
CultureMech_001844 --> ing7_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Na_acetate -.-> CHEBI_32954
ing8_Na_lactate["Na-lactate"]
CultureMech_001844 --> ing8_Na_lactate
CHEBI_75228["CHEBI:75228"]
ing8_Na_lactate -.-> CHEBI_75228
ing9_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
CultureMech_001844 --> ing9_2_Mercaptoethanesulfonate
CHEBI_17905["CHEBI:17905"]
ing9_2_Mercaptoethanesulfonate -.-> CHEBI_17905
ing10_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_001844 --> ing10_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing10_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001844 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001844 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001844 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_ZnCl2["ZnCl2"]
CultureMech_001844 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
CultureMech_001844 --> ing15_AlCl3_x_6_H2O
CHEBI_30115["CHEBI:30115"]
ing15_AlCl3_x_6_H2O -.-> CHEBI_30115
ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001844 --> ing16_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001844 --> ing17_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001844 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001844 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_H3BO3["H3BO3"]
CultureMech_001844 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001844 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
5.14563 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
5.63107 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.970874 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.38835 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.970874 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 | Yeast extract | 1.94175 G_PER_L | ||
| 7 | Trypticase peptone | 1.94175 G_PER_L | ||
| 8 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.776699 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 9 |
Na-lactate
C3H5O3.Na 112.06 g/mol
|
1.16505 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 10 |
2-Mercaptoethanesulfonate
C2H6O3S2 142.201 g/mol
|
0.485437 G_PER_L |
CHEBI:17905
coenzyme M
|
|
| 11 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
0.5 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.15 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
|
0.04 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
|
| 17 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.04 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 18 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- First, dissolve ferric citrate in water by heating and adjust to pH 7.5 with NaOH, then add other medium ingredients except acetate, lactate, and coenzyme M. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, lactate, and coenzyme M from sterile anoxic stock solutions prepared under 100% N2 gas.
- Prior to inoculation the pH of the complete medium should be checked and adjusted to 7.5, if necessary.
- First dissolve EDTA in distilled water and adjust pH to 7 using 2 N NaOH, then dissolve remaining compounds.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- bacillus_infernus_medium (CultureMech:001844)
- msa_fe_medium (CultureMech:006337)
- msa_fe_medium_replace_na_lactate_with_na_formate (CultureMech:006336)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.978314Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 705 |
| 2026-03-13T06:19:07.676215Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001844 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.015434+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BACILLUS INFERNUS MEDIUM" to "bacillus_infernus_medium" for consistent matching |
| 2026-06-05T07:40:50.261374+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.711510+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.589910+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: bacillus_infernus_medium, msa_fe_medium, msa_fe_medium_replace_na_lactate_with_na_formate |
| 2026-08-06T03:05:24.589954+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 365379a923e1940ab575e68bad53f19f49bcf587e689024eb06d0ebb77010ca6 |