bacillus_infernus_medium

CultureMech:001844 bacterial COMPLEX LIQUID
Original name: BACILLUS INFERNUS MEDIUM

Overview

External ontology
mediadive.medium:705 (source link unavailable) — BACILLUS INFERNUS MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium705.pdf

Composition

flowchart LR
    CultureMech_001844["bacillus_infernus_medium"]
    ing0_Ferric_citrate["Ferric citrate"]
    CultureMech_001844 --> ing0_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing0_Ferric_citrate -.-> CHEBI_144421
    ing1_NaCl["NaCl"]
    CultureMech_001844 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001844 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001844 --> ing3_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001844 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_001844 --> ing5_Yeast_extract
    ing6_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001844 --> ing6_Trypticase_peptone
    ing7_Na_acetate["Na-acetate"]
    CultureMech_001844 --> ing7_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Na_acetate -.-> CHEBI_32954
    ing8_Na_lactate["Na-lactate"]
    CultureMech_001844 --> ing8_Na_lactate
    CHEBI_75228["CHEBI:75228"]
    ing8_Na_lactate -.-> CHEBI_75228
    ing9_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
    CultureMech_001844 --> ing9_2_Mercaptoethanesulfonate
    CHEBI_17905["CHEBI:17905"]
    ing9_2_Mercaptoethanesulfonate -.-> CHEBI_17905
    ing10_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_001844 --> ing10_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing10_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001844 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001844 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001844 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_ZnCl2["ZnCl2"]
    CultureMech_001844 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
    CultureMech_001844 --> ing15_AlCl3_x_6_H2O
    CHEBI_30115["CHEBI:30115"]
    ing15_AlCl3_x_6_H2O -.-> CHEBI_30115
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001844 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001844 --> ing17_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001844 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001844 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_H3BO3["H3BO3"]
    CultureMech_001844 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001844 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Ferric citrate
C6H5FeO7 244.944 g/mol
5.14563 G_PER_L CHEBI:144421
iron(III) citrate
2 NaCl
Cl.Na 58.443 g/mol
5.63107 G_PER_L CHEBI:26710
sodium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
0.970874 G_PER_L CHEBI:31206
ammonium chloride
4 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.38835 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.970874 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 Yeast extract 1.94175 G_PER_L
7 Trypticase peptone 1.94175 G_PER_L
8 Na-acetate
C2H3O2.Na 82.034 g/mol
0.776699 G_PER_L CHEBI:32954
sodium acetate
9 Na-lactate
C3H5O3.Na 112.06 g/mol
1.16505 G_PER_L CHEBI:75228
sodium lactate
10 2-Mercaptoethanesulfonate
C2H6O3S2 142.201 g/mol
0.485437 G_PER_L CHEBI:17905
coenzyme M
11 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
0.5 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.15 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
16 AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
0.04 G_PER_L CHEBI:30115
aluminium trichloride hexahydrate
17 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.04 G_PER_L CHEBI:63939
sodium tungstate dihydrate
18 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.03 G_PER_L CHEBI:131361
disodium selenite pentahydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.02 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. First, dissolve ferric citrate in water by heating and adjust to pH 7.5 with NaOH, then add other medium ingredients except acetate, lactate, and coenzyme M. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, lactate, and coenzyme M from sterile anoxic stock solutions prepared under 100% N2 gas.
  2. Prior to inoculation the pH of the complete medium should be checked and adjusted to 7.5, if necessary.
  3. First dissolve EDTA in distilled water and adjust pH to 7 using 2 N NaOH, then dissolve remaining compounds.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.978314Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 705
2026-03-13T06:19:07.676215Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001844
2026-03-17T06:30:19.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.015434+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BACILLUS INFERNUS MEDIUM" to "bacillus_infernus_medium" for consistent matching
2026-06-05T07:40:50.261374+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.711510+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.