Overview
- External ontology
- mediadive.medium:732 (source link unavailable) — DEHALOBACTER RESTRICTUS MEDIUM (TCE)
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium732.pdf
Composition
flowchart LR
CultureMech_001867["dehalobacter_restrictus_medium_tce"]
ing0_K2HPO4["K2HPO4"]
CultureMech_001867 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
CultureMech_001867 --> ing1_NaH2PO4_x_H2O
CHEBI_37585["CHEBI:37585"]
ing1_NaH2PO4_x_H2O -.-> CHEBI_37585
ing2_Peptone["Peptone"]
CultureMech_001867 --> ing2_Peptone
ing3_Na_acetate["Na-acetate"]
CultureMech_001867 --> ing3_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Na_acetate -.-> CHEBI_32954
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_001867 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5__NH4_2CO3["(NH4)2CO3"]
CultureMech_001867 --> ing5__NH4_2CO3
ing6_NaHCO3["NaHCO3"]
CultureMech_001867 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001867 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001867 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001867 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Hexadecane["Hexadecane"]
CultureMech_001867 --> ing10_Hexadecane
CHEBI_45296["CHEBI:45296"]
ing10_Hexadecane -.-> CHEBI_45296
ing11_Tetrachloroethene["Tetrachloroethene"]
CultureMech_001867 --> ing11_Tetrachloroethene
CHEBI_17300["CHEBI:17300"]
ing11_Tetrachloroethene -.-> CHEBI_17300
ing12_Na2_EDTA["Na2-EDTA"]
CultureMech_001867 --> ing12_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing12_Na2_EDTA -.-> CHEBI_64734
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001867 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_ZnCl2["ZnCl2"]
CultureMech_001867 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001867 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_H3BO3["H3BO3"]
CultureMech_001867 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001867 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001867 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_AlCl3["AlCl3"]
CultureMech_001867 --> ing19_AlCl3
CHEBI_30114["CHEBI:30114"]
ing19_AlCl3 -.-> CHEBI_30114
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001867 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001867 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_Biotin["Biotin"]
CultureMech_001867 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_001867 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001867 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_001867 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_001867 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_001867 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001867 --> ing28_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_D_____pantothenate -.-> CHEBI_31345
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_001867 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
CultureMech_001867 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.747126 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
|
0.195402 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 3 | Peptone | 0.114943 G_PER_L | ||
| 4 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.528736 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000574713 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 | (NH4)2CO3 | 2.7 G_PER_L | ||
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
37.3 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
11 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
30 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Hexadecane
C16H34 226.448 g/mol
|
13.5 G_PER_L |
CHEBI:45296
hexadecane
|
|
| 12 |
Tetrachloroethene
C2Cl4 165.834 g/mol
|
1.5 G_PER_L |
CHEBI:17300
tetrachloroethene
|
|
| 13 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
2 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
AlCl3
AlCl3 133.341 g/mol
|
0.01 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.4 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
[Merged 2 duplicates: 0.1, 0.3] |
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.25 G_PER_L |
CHEBI:15940
nicotinic acid
|
[Merged 2 duplicates: 0.05, 0.2] |
| 29 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 | Vitamin B12 | 0.101 G_PER_L |
CHEBI:176843
vitamin B12
|
[Merged 2 duplicates: 0.001, 0.1] |
| 31 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.13 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
[Merged 2 duplicates: 0.05, 0.08] |
| 32 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 33 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 34 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 35 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Sparge solution A with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 9 ml medium in 50 ml bottle). Pressurize closed bottles with 80% H2 and 20% CO2 gas mixture to 0.5 bar overpressure and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C, D and F are autoclave under 100% N2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Prepare solution G by filling 13.5 ml hexadecane into a 50 ml serum bottle, then sparge with 100% N2 gas to make it anoxic and autoclave. Add 1.50 ml anoxic autoclaved tetrachloroethene to the sterile anoxic hexadecane solution by syringe. To complete the medium add appropriate amounts of solutions B to F to the sterile solution A in the sequence as indicated. The pH of the medium before inoculation should be at 7.2.
- Add solution G only after inoculation of the medium!
- First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- dehalobacter_restrictus_medium_tce (CultureMech:001867)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.066740Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 732 |
| 2026-02-06T05:10:12.761050Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:38.839455Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:07.933991Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001867 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.313236+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DEHALOBACTER RESTRICTUS MEDIUM (TCE)" to "dehalobacter_restrictus_medium_tce" for consistent matching |
| 2026-06-05T07:40:55.625541+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.206827+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:29.984570+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3ce890467cc3fd7d43841dbb7b5f3348e58bccd17a52cc55ac74832d581b6d8b |