dehalobacter_restrictus_medium_tce

Overview

External ontology

mediadive.medium:732 (source link unavailable) — DEHALOBACTER RESTRICTUS MEDIUM (TCE)

Applications

• Microbial cultivation

pH

7.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium732.pdf

Composition

flowchart LR
    CultureMech_001867["dehalobacter_restrictus_medium_tce"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001867 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_001867 --> ing1_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing1_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing2_Peptone["Peptone"]
    CultureMech_001867 --> ing2_Peptone
    ing3_Na_acetate["Na-acetate"]
    CultureMech_001867 --> ing3_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Na_acetate -.-> CHEBI_32954
    ing4_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001867 --> ing4_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Sodium_resazurin -.-> CHEBI_8806
    ing5__NH4_2CO3["(NH4)2CO3"]
    CultureMech_001867 --> ing5__NH4_2CO3
    ing6_NaHCO3["NaHCO3"]
    CultureMech_001867 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001867 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001867 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001867 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Hexadecane["Hexadecane"]
    CultureMech_001867 --> ing10_Hexadecane
    CHEBI_45296["CHEBI:45296"]
    ing10_Hexadecane -.-> CHEBI_45296
    ing11_Tetrachloroethene["Tetrachloroethene"]
    CultureMech_001867 --> ing11_Tetrachloroethene
    CHEBI_17300["CHEBI:17300"]
    ing11_Tetrachloroethene -.-> CHEBI_17300
    ing12_Na2_EDTA["Na2-EDTA"]
    CultureMech_001867 --> ing12_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing12_Na2_EDTA -.-> CHEBI_64734
    ing13_ZnCl2["ZnCl2"]
    CultureMech_001867 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001867 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_001867 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001867 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001867 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_AlCl3["AlCl3"]
    CultureMech_001867 --> ing18_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing18_AlCl3 -.-> CHEBI_30114
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001867 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001867 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_Biotin["Biotin"]
    CultureMech_001867 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_001867 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001867 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_001867 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001867 --> ing25_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing26__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_001867 --> ing26__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing27_D_____biotin["D-(+)-biotin"]
    CultureMech_001867 --> ing27_D_____biotin
    ing27_D_____biotin -.-> CHEBI_15956
    ing28_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001867 --> ing28_Calcium_pantothenate
    ing28_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (29)

Preparation

1. Sparge solution A with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 9 ml medium in 50 ml bottle). Pressurize closed bottles with 80% H2 and 20% CO2 gas mixture to 0.5 bar overpressure and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C, D and F are autoclave under 100% N2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Prepare solution G by filling 13.5 ml hexadecane into a 50 ml serum bottle, then sparge with 100% N2 gas to make it anoxic and autoclave. Add 1.50 ml anoxic autoclaved tetrachloroethene to the sterile anoxic hexadecane solution by syringe. To complete the medium add appropriate amounts of solutions B to F to the sterile solution A in the sequence as indicated. The pH of the medium before inoculation should be at 7.2.
2. Add solution G only after inoculation of the medium!
3. First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.

Solutions

Target organisms

No target organisms are recorded.

Provenance