Overview
- External ontology
- mediadive.medium:772 (source link unavailable) — PARACOCCUS ALCALIPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium772.pdf
Composition
flowchart LR
CultureMech_001906["paracoccus_alcaliphilus_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_001906 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_001906 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Na2HPO4["Na2HPO4"]
CultureMech_001906 --> ing2_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing2_Na2HPO4 -.-> CHEBI_34683
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001906 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Fe_III__citrate["Fe(III) citrate"]
CultureMech_001906 --> ing4_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing4_Fe_III__citrate -.-> CHEBI_144421
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001906 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001906 --> ing6_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001906 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_CuSO4_x_2_H2O["CuSO4 x 2 H2O"]
CultureMech_001906 --> ing8_CuSO4_x_2_H2O
CHEBI_23414["CHEBI:23414"]
ing8_CuSO4_x_2_H2O -.-> CHEBI_23414
ing9_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001906 --> ing9_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing9_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing10_Methanol["Methanol"]
CultureMech_001906 --> ing10_Methanol
CHEBI_17790["CHEBI:17790"]
ing10_Methanol -.-> CHEBI_17790
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
3 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.03 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.03 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.005 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.005 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
CuSO4 x 2 H2O
Cu.O4S 159.609 g/mol
|
0.0005 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
|
| 10 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.0004 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 11 |
Methanol
CH4O 32.042 g/mol
|
7.92 G_PER_L |
CHEBI:17790
methanol
|
Preparation
- Thiamine hydrochloride may be replaced by 2 g/l yeast extract. The pH of the medium is adjusted to pH 9.0 with filter sterilised 10% Na2CO3. 10 ml sterile methanol (filter sterilised, or autoclaved in crimp top sealed bottles) is added to the sterilised medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- paracoccus_alcaliphilus_medium (CultureMech:001906)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.196226Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 772 |
| 2026-03-13T06:19:08.310730Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001906 |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.739561+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PARACOCCUS ALCALIPHILUS MEDIUM" to "paracoccus_alcaliphilus_medium" for consistent matching |
| 2026-06-05T07:41:40.862163+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.873357+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.232417+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0f81fc1fb75432dcab7957853b23d8bfb60d28ef4d6157083c00d39a1ed0901d |