paracoccus_alcaliphilus_medium

CultureMech:001906 bacterial DEFINED LIQUID
Original name: PARACOCCUS ALCALIPHILUS MEDIUM

Overview

External ontology
mediadive.medium:772 (source link unavailable) — PARACOCCUS ALCALIPHILUS MEDIUM
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium772.pdf

Composition

flowchart LR
    CultureMech_001906["paracoccus_alcaliphilus_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001906 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001906 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_001906 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001906 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_001906 --> ing4_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing4_Fe_III__citrate -.-> CHEBI_144421
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001906 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001906 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001906 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_CuSO4_x_2_H2O["CuSO4 x 2 H2O"]
    CultureMech_001906 --> ing8_CuSO4_x_2_H2O
    CHEBI_23414["CHEBI:23414"]
    ing8_CuSO4_x_2_H2O -.-> CHEBI_23414
    ing9_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001906 --> ing9_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing9_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing10_Methanol["Methanol"]
    CultureMech_001906 --> ing10_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing10_Methanol -.-> CHEBI_17790

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Na2HPO4
HO4P.2Na 141.958 g/mol
3 G_PER_L CHEBI:34683
disodium hydrogenphosphate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Fe(III) citrate
C6H5FeO7 244.944 g/mol
0.03 G_PER_L CHEBI:144421
iron(III) citrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.03 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.005 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.005 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 CuSO4 x 2 H2O
Cu.O4S 159.609 g/mol
0.0005 G_PER_L CHEBI:23414
copper(II) sulfate
10 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.0004 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
11 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol

Preparation

  1. Thiamine hydrochloride may be replaced by 2 g/l yeast extract. The pH of the medium is adjusted to pH 9.0 with filter sterilised 10% Na2CO3. 10 ml sterile methanol (filter sterilised, or autoclaved in crimp top sealed bottles) is added to the sterilised medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.196226Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 772
2026-03-13T06:19:08.310730Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001906
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.739561+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PARACOCCUS ALCALIPHILUS MEDIUM" to "paracoccus_alcaliphilus_medium" for consistent matching
2026-06-05T07:41:40.862163+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.873357+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.