Overview
- External ontology
- mediadive.medium:792 (source link unavailable) — PYROLOBUS FUMARII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium792.pdf
Composition
flowchart LR
CultureMech_001932["pyrolobus_fumarii_medium"]
ing0_NaCl["NaCl"]
CultureMech_001932 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001932 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001932 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001932 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_001932 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaBr["NaBr"]
CultureMech_001932 --> ing5_NaBr
CHEBI_63004["CHEBI:63004"]
ing5_NaBr -.-> CHEBI_63004
ing6_H3BO3["H3BO3"]
CultureMech_001932 --> ing6_H3BO3
CHEBI_33118["CHEBI:33118"]
ing6_H3BO3 -.-> CHEBI_33118
ing7_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001932 --> ing7_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing7_SrCl2_x_6_H2O -.-> CHEBI_36385
ing8_KI["KI"]
CultureMech_001932 --> ing8_KI
CHEBI_8346["CHEBI:8346"]
ing8_KI -.-> CHEBI_8346
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_001932 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Double_distilled_water["Double distilled water"]
CultureMech_001932 --> ing10_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing10_Double_distilled_water -.-> CHEBI_15377
ing11_KNO3["KNO3"]
CultureMech_001932 --> ing11_KNO3
CHEBI_63043["CHEBI:63043"]
ing11_KNO3 -.-> CHEBI_63043
ing12_KH2PO4["KH2PO4"]
CultureMech_001932 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001932 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001932 --> ing14_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing14_MnSO4_x_H2O -.-> CHEBI_86364
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001932 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001932 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001932 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001932 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001932 --> ing19_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001932 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_001932 --> ing21__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing21__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001932 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing23_Na2SeO4["Na2SeO4"]
CultureMech_001932 --> ing23_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing23_Na2SeO4 -.-> CHEBI_77775
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_001932 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001932 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26_D_____biotin["D-(+)-biotin"]
CultureMech_001932 --> ing26_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing26_D_____biotin -.-> CHEBI_15956
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_001932 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001932 --> ing28_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_pantothenate -.-> CHEBI_31345
ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001932 --> ing29_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
CultureMech_001932 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
24.003999999999998 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 14.004, 10.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
33.53893 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.53893, 30.0] |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.78059 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.384227 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.384227, 1.0] |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.33367 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaBr
Br.Na 102.894 g/mol
|
0.0505561 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 7 |
H3BO3
H3BO3 61.833 g/mol
|
0.1151668 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.0151668, 0.1] |
| 8 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.00707786 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 9 |
KI
I.K 166.002 g/mol
|
5.05561e-05 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000505561 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Double distilled water
H2O 18.015 g/mol
|
980 G_PER_L |
CHEBI:15377
water
|
|
| 12 |
KNO3
K.NO3 101.102 g/mol
|
100 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
50 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
15 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 24 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 25 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 27 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 31 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Sparge solution A with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 20 ml medium in 100 ml serum bottles) and autoclave. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere and solution C is prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B, C and D are added to solution A. Adjust pH of the complete medium to 5.8 using a sterile anoxic stock solution of 5% (w/v) Na2CO3, if necessary.
- After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- Note: Solution A has to be prepared freshly. It cannot be stored for a long period of time.
- First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pyrolobus_fumarii_medium (CultureMech:001932)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.253674Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 792 |
| 2026-03-13T03:08:40.691363Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:08.556433Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001932 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.010259+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYROLOBUS FUMARII MEDIUM" to "pyrolobus_fumarii_medium" for consistent matching |
| 2026-06-05T07:41:43.465459+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.782488+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:10.728022+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.649305+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0aa02c21be087785ade5bc5c004f766a9494486cb19d852fe0b902ccf8e687a9 |