pyrolobus_fumarii_medium

CultureMech:001932 archaea DEFINED LIQUID
Original name: PYROLOBUS FUMARII MEDIUM

Overview

External ontology
mediadive.medium:792 (source link unavailable) — PYROLOBUS FUMARII MEDIUM
Applications
  • Microbial cultivation
pH
5.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium792.pdf

Composition

flowchart LR
    CultureMech_001932["pyrolobus_fumarii_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001932 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001932 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001932 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001932 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_001932 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaBr["NaBr"]
    CultureMech_001932 --> ing5_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing5_NaBr -.-> CHEBI_63004
    ing6_H3BO3["H3BO3"]
    CultureMech_001932 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001932 --> ing7_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing7_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing8_KI["KI"]
    CultureMech_001932 --> ing8_KI
    CHEBI_8346["CHEBI:8346"]
    ing8_KI -.-> CHEBI_8346
    ing9_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001932 --> ing9_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Sodium_resazurin -.-> CHEBI_8806
    ing10_Double_distilled_water["Double distilled water"]
    CultureMech_001932 --> ing10_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing10_Double_distilled_water -.-> CHEBI_15377
    ing11_KNO3["KNO3"]
    CultureMech_001932 --> ing11_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing11_KNO3 -.-> CHEBI_63043
    ing12_KH2PO4["KH2PO4"]
    CultureMech_001932 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001932 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001932 --> ing14_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing15_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001932 --> ing15_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing15_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001932 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_001932 --> ing17__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing17__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001932 --> ing18_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing19_Na2SeO4["Na2SeO4"]
    CultureMech_001932 --> ing19_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing19_Na2SeO4 -.-> CHEBI_77775
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001932 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_D_____biotin["D-(+)-biotin"]
    CultureMech_001932 --> ing21_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_D_____biotin -.-> CHEBI_15956
    ing22_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001932 --> ing22_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
24.003999999999998 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 14.004, 10.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
33.53893 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.53893, 30.0]
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.78059 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.384227 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.384227, 1.0]
5 KCl
Cl.K 74.551 g/mol
0.33367 G_PER_L CHEBI:32588
potassium chloride
6 NaBr
Br.Na 102.894 g/mol
0.0505561 G_PER_L CHEBI:63004
sodium bromide
7 H3BO3
H3BO3 61.833 g/mol
0.1151668 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.0151668, 0.1]
8 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.00707786 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
9 KI
I.K 166.002 g/mol
5.05561e-05 G_PER_L CHEBI:8346
potassium iodide
10 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000505561 G_PER_L CHEBI:8806
Resazurin
11 Double distilled water
H2O 18.015 g/mol
980 G_PER_L CHEBI:15377
water
12 KNO3
K.NO3 101.102 g/mol
100 G_PER_L CHEBI:63043
potassium nitrate
13 KH2PO4
H2O4P.K 136.084 g/mol
50 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
15 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
16 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.18 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2.8 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
19 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
20 Na2SeO4
2Na.O4Se 188.936 g/mol
0.1 G_PER_L CHEBI:77775
sodium selenate
21 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
23 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Sparge solution A with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 20 ml medium in 100 ml serum bottles) and autoclave. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere and solution C is prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B, C and D are added to solution A. Adjust pH of the complete medium to 5.8 using a sterile anoxic stock solution of 5% (w/v) Na2CO3, if necessary.
  2. After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
  3. Note: Solution A has to be prepared freshly. It cannot be stored for a long period of time.
  4. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Solutions

Wolfe's mineral elixir

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
MnSO4 x H2O 5 G_PER_L
FeSO4 x 7 H2O 1 G_PER_L
CoCl2 x 6 H2O 1.8 G_PER_L
ZnSO4 x 7 H2O 1.8 G_PER_L

Seven vitamins solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.1 G_PER_L
Nicotinic acid 0.2 G_PER_L
Pyridoxine hydrochloride 0.3 G_PER_L
Thiamine-HCl x 2 H2O 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.253674Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 792
2026-03-13T03:08:40.691363Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:08.556433Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001932
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.010259+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYROLOBUS FUMARII MEDIUM" to "pyrolobus_fumarii_medium" for consistent matching
2026-06-05T07:41:43.465459+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.782488+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:10.728022+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.484831+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 8 stock-strength component(s) out of ingredients into 2 solution(s): 4 -> "Wolfe's mineral elixir" @ 1 ml/l; 4 -> 'Seven vitamins solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).