helmutkoenigia_medium

CultureMech:001999 bacterial COMPLEX LIQUID
Original name: HELMUTKOENIGIA MEDIUM

Overview

External ontology
mediadive.medium:843 (source link unavailable) — HELMUTKOENIGIA MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'for_dsm_13862', 'source': 'KOMODO', 'source_id': 'komodo.medium:843.1', 'original_category': 'bacterial'}
  • {'name': 'medium_843_modified_for_dsm_18056', 'source': 'KOMODO', 'source_id': 'komodo.medium:843_18056', 'original_category': 'bacterial'}
  • {'name': 'treponema_zas_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:843', 'original_category': 'bacterial'}
pH range
{'min': 7.2, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium843.pdf

Composition

flowchart LR
    CultureMech_001999["helmutkoenigia_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001999 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_001999 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001999 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001999 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_001999 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KH2PO4["KH2PO4"]
    CultureMech_001999 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Na2SO4["Na2SO4"]
    CultureMech_001999 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001999 --> ing7_Trypticase_peptone
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001999 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Na2CO3["Na2CO3"]
    CultureMech_001999 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_DL_Dithiothreitol["DL-Dithiothreitol"]
    CultureMech_001999 --> ing10_DL_Dithiothreitol
    CHEBI_18320["CHEBI:18320"]
    ing10_DL_Dithiothreitol -.-> CHEBI_18320
    ing11_Cellobiose["Cellobiose"]
    CultureMech_001999 --> ing11_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing11_Cellobiose -.-> CHEBI_17057
    ing12_Sucrose["Sucrose"]
    CultureMech_001999 --> ing12_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing12_Sucrose -.-> CHEBI_17992
    ing13_Maltose_x_H2O["Maltose x H2O"]
    CultureMech_001999 --> ing13_Maltose_x_H2O
    CHEBI_17306["CHEBI:17306"]
    ing13_Maltose_x_H2O -.-> CHEBI_17306
    ing14_Trehalose["Trehalose"]
    CultureMech_001999 --> ing14_Trehalose
    CHEBI_27082["CHEBI:27082"]
    ing14_Trehalose -.-> CHEBI_27082
    ing15_D_Xylose["D-Xylose"]
    CultureMech_001999 --> ing15_D_Xylose
    CHEBI_65327["CHEBI:65327"]
    ing15_D_Xylose -.-> CHEBI_65327
    ing16_Yeast_extract["Yeast extract"]
    CultureMech_001999 --> ing16_Yeast_extract
    ing17_Thiamine_pyrophosphate["Thiamine pyrophosphate"]
    CultureMech_001999 --> ing17_Thiamine_pyrophosphate
    CHEBI_18290["CHEBI:18290"]
    ing17_Thiamine_pyrophosphate -.-> CHEBI_18290
    ing18_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
    CultureMech_001999 --> ing18_Pyridoxal_hydrochloride
    CHEBI_131529["CHEBI:131529"]
    ing18_Pyridoxal_hydrochloride -.-> CHEBI_131529
    ing19_Pyridoxal_phosphate["Pyridoxal phosphate"]
    CultureMech_001999 --> ing19_Pyridoxal_phosphate
    CHEBI_18405["CHEBI:18405"]
    ing19_Pyridoxal_phosphate -.-> CHEBI_18405
    ing20_Ca_folinate["Ca-folinate"]
    CultureMech_001999 --> ing20_Ca_folinate
    CHEBI_31340["CHEBI:31340"]
    ing20_Ca_folinate -.-> CHEBI_31340
    ing21___NAD["ß-NAD"]
    CultureMech_001999 --> ing21___NAD
    ing22_Coenzyme_A["Coenzyme A"]
    CultureMech_001999 --> ing22_Coenzyme_A
    CHEBI_15346["CHEBI:15346"]
    ing22_Coenzyme_A -.-> CHEBI_15346
    ing23_FAD["FAD"]
    CultureMech_001999 --> ing23_FAD
    CHEBI_16238["CHEBI:16238"]
    ing23_FAD -.-> CHEBI_16238
    ing24_Haemin["Haemin"]
    CultureMech_001999 --> ing24_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing24_Haemin -.-> CHEBI_50385
    ing25_Nicotinamide["Nicotinamide"]
    CultureMech_001999 --> ing25_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing25_Nicotinamide -.-> CHEBI_17154
    ing26_Folic_acid["Folic acid"]
    CultureMech_001999 --> ing26_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing26_Folic_acid -.-> CHEBI_27470
    ing27_Riboflavin["Riboflavin"]
    CultureMech_001999 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_HCl["HCl"]
    CultureMech_001999 --> ing28_HCl
    CHEBI_17883["CHEBI:17883"]
    ing28_HCl -.-> CHEBI_17883
    ing29_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001999 --> ing29_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing29_FeCl2_x_4_H2O -.-> CHEBI_86249
    CultureMech_001999 --> more["...17 more"]

Ingredients (47)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
0.997009 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.498504 G_PER_L CHEBI:32588
potassium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.398804 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0997009 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.299103 G_PER_L CHEBI:31206
ammonium chloride
6 KH2PO4
H2O4P.K 136.084 g/mol
0.199402 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Na2SO4
2Na.O4S 142.043 g/mol
0.149551 G_PER_L CHEBI:32149
sodium sulfate
8 Trypticase peptone 0.997009 G_PER_L
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000498504 G_PER_L CHEBI:8806
Resazurin
10 Na2CO3
CO3.2Na 105.988 g/mol
1.49551 G_PER_L CHEBI:29377
sodium carbonate
11 DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
0.398804 G_PER_L CHEBI:18320
1,4-dithiothreitol
12 Cellobiose
C12H22O11 342.297 g/mol
56.7 G_PER_L CHEBI:17057
cellobiose
13 Sucrose
C12H22O11 342.297 g/mol
56.7 G_PER_L CHEBI:17992
sucrose
14 Maltose x H2O
C12H22O11 342.297 g/mol
60 G_PER_L CHEBI:17306
maltose
15 Trehalose
C12H22O11 342.297 g/mol
63.3 G_PER_L CHEBI:27082
trehalose
16 D-Xylose
C5H10O5 150.13 g/mol
25 G_PER_L CHEBI:65327
D-xylose
17 Yeast extract 280 G_PER_L
18 Thiamine pyrophosphate
C12H19N4O7P2S.Cl 460.773 g/mol
2.5 G_PER_L CHEBI:18290
thiamine(1+) diphosphate chloride
19 Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
0.25 G_PER_L CHEBI:131529
pyridoxal hydrochloride
20 Pyridoxal phosphate
C8H10NO6P 247.143 g/mol
0.25 G_PER_L CHEBI:18405
pyridoxal 5'-phosphate
21 Ca-folinate
C20H21N7O7.Ca 511.508 g/mol
0.05 G_PER_L CHEBI:31340
Calcium folinate
22 ß-NAD 0.05 G_PER_L
23 Coenzyme A
C21H36N7O16P3S 767.541 g/mol
0.05 G_PER_L CHEBI:15346
coenzyme A
24 FAD
C27H33N9O15P2 785.557 g/mol
0.05 G_PER_L CHEBI:16238
FAD
25 Haemin
C34H32ClFeN4O4 651.952 g/mol
0.065 G_PER_L CHEBI:50385
hemin
26 Nicotinamide
C6H6N2O 122.127 g/mol
0.025 G_PER_L CHEBI:17154
nicotinamide
27 Folic acid
C19H19N7O6 441.404 g/mol
0.0025 G_PER_L CHEBI:27470
folic acid
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.0005 G_PER_L CHEBI:17015
riboflavin
29 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
30 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
31 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
32 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
33 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
34 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
35 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
36 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
37 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
38 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
39 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
40 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
41 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
42 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
43 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
44 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
45 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
46 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.3 G_PER_L CHEBI:30961
pyridoxine hydrochloride
47 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.2 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. Dissolve ingredients (except carbonate, sugar mix, yeast autolysate, vitamins, co-factors, and DTT), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume (e.g., 4.5 ml per Hungate-type tube) and autoclave. After sterilization add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and sugar mix, yeast autolysate, vitamins, co-factors and DTT from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration. Check pH of complete medium and adjust to 7.2 - 7.4, if necessary.
  2. Note: Basal medium and yeast autolysate have to be prepared freshly and cannot be stored for longer periods.
  3. Suspend dry baker's yeast (SIGMA YSC-1) in distilled water and allow cells to autolyse for 48 h at 55°C. Thereafter, cell debris are removed by centrifugation (3.700 x g for 30 min) and the amber-colored supernatant is stored in aliquots at -20°C.
  4. Adjust pH to 7.0 with 1 N NaOH against indicator paper, filter sterilize and store under 100% N2 gas atmosphere.
  5. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:12.470489Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 843
2026-03-13T06:19:09.264631Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001999
2026-03-17T06:30:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.800230+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HELMUTKOENIGIA MEDIUM" to "helmutkoenigia_medium" for consistent matching
2026-05-20T06:01:22.501683+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4}
2026-06-05T07:41:08.397263+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 43 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.724020+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:34.516889+00:00 recipe-merger Merged 4 duplicate recipes into canonical record Sources: for_dsm_13862, helmutkoenigia_medium, medium_843_modified_for_dsm_18056, treponema_zas_medium
2026-08-06T03:05:34.516955+00:00 merge_recipes.py MERGED_RECIPES merged 4 source recipe(s) on fingerprint 50fccda7e2276e98100b145866b78c6b28d71fff7d2c51229016536603296d5b