Overview
- External ontology
- komodo.medium:843 (source link unavailable) — TREPONEMA ZAS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.4}
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 843 | DSMZ Medium: 843 (mediadive.medium:843) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006648["treponema_zas_medium"]
ing0_NaCl["NaCl"]
CultureMech_006648 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_006648 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006648 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006648 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_006648 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KH2PO4["KH2PO4"]
CultureMech_006648 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Na2SO4["Na2SO4"]
CultureMech_006648 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_Trypticase_peptone["Trypticase peptone"]
CultureMech_006648 --> ing7_Trypticase_peptone
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_006648 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_Na2CO3["Na2CO3"]
CultureMech_006648 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_DL_Dithiothreitol["DL-Dithiothreitol"]
CultureMech_006648 --> ing10_DL_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing10_DL_Dithiothreitol -.-> CHEBI_18320
ing11_Cellobiose["Cellobiose"]
CultureMech_006648 --> ing11_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing11_Cellobiose -.-> CHEBI_17057
ing12_Sucrose["Sucrose"]
CultureMech_006648 --> ing12_Sucrose
CHEBI_17992["CHEBI:17992"]
ing12_Sucrose -.-> CHEBI_17992
ing13_Maltose_x_H2O["Maltose x H2O"]
CultureMech_006648 --> ing13_Maltose_x_H2O
CHEBI_17306["CHEBI:17306"]
ing13_Maltose_x_H2O -.-> CHEBI_17306
ing14_Trehalose["Trehalose"]
CultureMech_006648 --> ing14_Trehalose
CHEBI_27082["CHEBI:27082"]
ing14_Trehalose -.-> CHEBI_27082
ing15_D_Xylose["D-Xylose"]
CultureMech_006648 --> ing15_D_Xylose
CHEBI_65327["CHEBI:65327"]
ing15_D_Xylose -.-> CHEBI_65327
ing16_Yeast_extract["Yeast extract"]
CultureMech_006648 --> ing16_Yeast_extract
ing17_Thiamine_pyrophosphate["Thiamine pyrophosphate"]
CultureMech_006648 --> ing17_Thiamine_pyrophosphate
CHEBI_18290["CHEBI:18290"]
ing17_Thiamine_pyrophosphate -.-> CHEBI_18290
ing18_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
CultureMech_006648 --> ing18_Pyridoxal_hydrochloride
CHEBI_131529["CHEBI:131529"]
ing18_Pyridoxal_hydrochloride -.-> CHEBI_131529
ing19_Pyridoxal_phosphate["Pyridoxal phosphate"]
CultureMech_006648 --> ing19_Pyridoxal_phosphate
CHEBI_18405["CHEBI:18405"]
ing19_Pyridoxal_phosphate -.-> CHEBI_18405
ing20_Ca_folinate["Ca-folinate"]
CultureMech_006648 --> ing20_Ca_folinate
CHEBI_31340["CHEBI:31340"]
ing20_Ca_folinate -.-> CHEBI_31340
ing21___NAD["ß-NAD"]
CultureMech_006648 --> ing21___NAD
ing22_Coenzyme_A["Coenzyme A"]
CultureMech_006648 --> ing22_Coenzyme_A
CHEBI_15346["CHEBI:15346"]
ing22_Coenzyme_A -.-> CHEBI_15346
ing23_FAD["FAD"]
CultureMech_006648 --> ing23_FAD
CHEBI_16238["CHEBI:16238"]
ing23_FAD -.-> CHEBI_16238
ing24_Haemin["Haemin"]
CultureMech_006648 --> ing24_Haemin
CHEBI_50385["CHEBI:50385"]
ing24_Haemin -.-> CHEBI_50385
ing25_Nicotinamide["Nicotinamide"]
CultureMech_006648 --> ing25_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing25_Nicotinamide -.-> CHEBI_17154
ing26_Folic_acid["Folic acid"]
CultureMech_006648 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Riboflavin["Riboflavin"]
CultureMech_006648 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_HCl["HCl"]
CultureMech_006648 --> ing28_HCl
CHEBI_17883["CHEBI:17883"]
ing28_HCl -.-> CHEBI_17883
ing29_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_006648 --> ing29_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing29_FeCl2_x_4_H2O -.-> CHEBI_86249
CultureMech_006648 --> more["...17 more"]
Ingredients (47)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.997009 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.498504 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.398804 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0997009 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.299103 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.199402 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.149551 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 | Trypticase peptone | 0.997009 G_PER_L | ||
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000498504 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.49551 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 11 |
DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
|
0.398804 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 12 |
Cellobiose
C12H22O11 342.297 g/mol
|
56.7 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 13 |
Sucrose
C12H22O11 342.297 g/mol
|
56.7 G_PER_L |
CHEBI:17992
sucrose
|
|
| 14 |
Maltose x H2O
C12H22O11 342.297 g/mol
|
60 G_PER_L |
CHEBI:17306
maltose
|
|
| 15 |
Trehalose
C12H22O11 342.297 g/mol
|
63.3 G_PER_L |
CHEBI:27082
trehalose
|
|
| 16 |
D-Xylose
C5H10O5 150.13 g/mol
|
25 G_PER_L |
CHEBI:65327
D-xylose
|
|
| 17 | Yeast extract | 280 G_PER_L | ||
| 18 |
Thiamine pyrophosphate
C12H19N4O7P2S.Cl 460.773 g/mol
|
2.5 G_PER_L |
CHEBI:18290
thiamine(1+) diphosphate chloride
|
|
| 19 |
Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
|
0.25 G_PER_L |
CHEBI:131529
pyridoxal hydrochloride
|
|
| 20 |
Pyridoxal phosphate
C8H10NO6P 247.143 g/mol
|
0.25 G_PER_L |
CHEBI:18405
pyridoxal 5'-phosphate
|
|
| 21 |
Ca-folinate
C20H21N7O7.Ca 511.508 g/mol
|
0.05 G_PER_L |
CHEBI:31340
Calcium folinate
|
|
| 22 | ß-NAD | 0.05 G_PER_L | ||
| 23 |
Coenzyme A
C21H36N7O16P3S 767.541 g/mol
|
0.05 G_PER_L |
CHEBI:15346
coenzyme A
|
|
| 24 |
FAD
C27H33N9O15P2 785.557 g/mol
|
0.05 G_PER_L |
CHEBI:16238
FAD
|
|
| 25 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.065 G_PER_L |
CHEBI:50385
hemin
|
|
| 26 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0025 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.0005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 30 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 31 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 32 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 33 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 34 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 35 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 36 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 37 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 38 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 39 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 40 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 41 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 42 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 43 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 44 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 45 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 46 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 47 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 843 |
| 2026-02-03T11:18:56.034438 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 843 |
| 2026-03-13T06:19:42.673296Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006648 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:00.336018+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "TREPONEMA ZAS MEDIUM" to "treponema_zas_medium" for consistent matching |
| 2026-05-20T06:02:11.017211+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4} |
| 2026-06-05T07:41:54.171403+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 43 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:14.308327+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_843_helmutkoenigia_score10.py | RESOLVED_KOMODO_843_HELMUTKOENIGIA_TOPOLOGY | KOMODO Medium 843 has the same 47-ingredient signature as DSMZ Medium 843. |