bacto_middlebrook_7h9_broth

Overview

External ontology

mediadive.medium:857 (source link unavailable) — BACTO MIDDLEBROOK 7H9 BROTH

Applications

• Microbial cultivation

pH range

{'min': 6.4, 'max': 6.8}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium857.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_002016["bacto_middlebrook_7h9_broth"]
    ing0_Ammonium_Sulfate["Ammonium Sulfate"]
    CultureMech_002016 --> ing0_Ammonium_Sulfate
    CHEBI_62946["CHEBI:62946"]
    ing0_Ammonium_Sulfate -.-> CHEBI_62946
    ing1_Monopotassium_Phosphate["Monopotassium Phosphate"]
    CultureMech_002016 --> ing1_Monopotassium_Phosphate
    CHEBI_63036["CHEBI:63036"]
    ing1_Monopotassium_Phosphate -.-> CHEBI_63036
    ing2_Disodium_Phosphate["Disodium Phosphate"]
    CultureMech_002016 --> ing2_Disodium_Phosphate
    CHEBI_34683["CHEBI:34683"]
    ing2_Disodium_Phosphate -.-> CHEBI_34683
    ing3_Sodium_citrate["Sodium citrate"]
    CultureMech_002016 --> ing3_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Sodium_citrate -.-> CHEBI_53258
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_002016 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Calcium_Chloride["Calcium Chloride"]
    CultureMech_002016 --> ing5_Calcium_Chloride
    CHEBI_3312["CHEBI:3312"]
    ing5_Calcium_Chloride -.-> CHEBI_3312
    ing6_Zinc_Sulfate["Zinc Sulfate"]
    CultureMech_002016 --> ing6_Zinc_Sulfate
    CHEBI_35176["CHEBI:35176"]
    ing6_Zinc_Sulfate -.-> CHEBI_35176
    ing7_Copper_Sulfate["Copper Sulfate"]
    CultureMech_002016 --> ing7_Copper_Sulfate
    CHEBI_23414["CHEBI:23414"]
    ing7_Copper_Sulfate -.-> CHEBI_23414
    ing8_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_002016 --> ing8_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing8_L_Glutamic_acid -.-> CHEBI_16015
    ing9_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_002016 --> ing9_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing9_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing10_Pyridoxine["Pyridoxine"]
    CultureMech_002016 --> ing10_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing10_Pyridoxine -.-> CHEBI_16709
    ing11_Biotin["Biotin"]
    CultureMech_002016 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Tryptone["Tryptone"]
    CultureMech_002016 --> ing12_Tryptone
    ing13_Yeast_extract["Yeast extract"]
    CultureMech_002016 --> ing13_Yeast_extract
    ing14_Sodium_chloride["Sodium chloride"]
    CultureMech_002016 --> ing14_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing14_Sodium_chloride -.-> CHEBI_26710
    ing15_Dextrose["Dextrose"]
    CultureMech_002016 --> ing15_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing15_Dextrose -.-> CHEBI_17634
    ing16_Catalase["Catalase"]
    CultureMech_002016 --> ing16_Catalase

Ingredients (17)

Preparation

1. Solution g/liter (final solution) 4.7 g/l. Final pH at 25°C 6.6 ± 0.2.
2. To prepare one liter of final medium, suspend the amount of dehydrated powder indicated under FORMULAE-Solution (and on the package label) in 900 ml distilled and deionized water containing 2 ml glycerol and heat to boiling to dissolve completely. Sterilize in the autoclave for 10 or 15 min at 15 Ibs pressure (121°C), per label copy. Cool to 50-55°C.
3. Aseptically add 100 ml of the appropriate ADC Enrichment and mix throughly.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• bacto_middlebrook_7h9_broth (CultureMech:002016)