bacto_middlebrook_7h9_broth

CultureMech:002016 bacterial COMPLEX LIQUID
Original name: BACTO MIDDLEBROOK 7H9 BROTH

Overview

External ontology
mediadive.medium:857 (source link unavailable) — BACTO MIDDLEBROOK 7H9 BROTH
Applications
  • Microbial cultivation
pH range
{'min': 6.4, 'max': 6.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium857.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. False LB constituent expansion is withdrawn. Source-specific amounts and preparation boundaries supersede flattened concentrations. Existing chemical annotations and earlier history are preserved, not recertified; no organism-growth association is added. Original DSMZ 857 protocol explicitly prepares one liter final medium with 900 ml water and 2 ml glycerol, then adds 100 ml ADC after cooling. Do not autoclave the added enrichment or replace its 0.3 mg catalase with another recipe's OADC amount.

Composition

flowchart LR
    CultureMech_002016["bacto_middlebrook_7h9_broth"]
    ing0_Ammonium_Sulfate["Ammonium Sulfate"]
    CultureMech_002016 --> ing0_Ammonium_Sulfate
    CHEBI_62946["CHEBI:62946"]
    ing0_Ammonium_Sulfate -.-> CHEBI_62946
    ing1_Monopotassium_Phosphate["Monopotassium Phosphate"]
    CultureMech_002016 --> ing1_Monopotassium_Phosphate
    CHEBI_63036["CHEBI:63036"]
    ing1_Monopotassium_Phosphate -.-> CHEBI_63036
    ing2_Disodium_Phosphate["Disodium Phosphate"]
    CultureMech_002016 --> ing2_Disodium_Phosphate
    CHEBI_34683["CHEBI:34683"]
    ing2_Disodium_Phosphate -.-> CHEBI_34683
    ing3_Sodium_citrate["Sodium citrate"]
    CultureMech_002016 --> ing3_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Sodium_citrate -.-> CHEBI_53258
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_002016 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Calcium_Chloride["Calcium Chloride"]
    CultureMech_002016 --> ing5_Calcium_Chloride
    CHEBI_3312["CHEBI:3312"]
    ing5_Calcium_Chloride -.-> CHEBI_3312
    ing6_Zinc_Sulfate["Zinc Sulfate"]
    CultureMech_002016 --> ing6_Zinc_Sulfate
    CHEBI_35176["CHEBI:35176"]
    ing6_Zinc_Sulfate -.-> CHEBI_35176
    ing7_Copper_Sulfate["Copper Sulfate"]
    CultureMech_002016 --> ing7_Copper_Sulfate
    CHEBI_23414["CHEBI:23414"]
    ing7_Copper_Sulfate -.-> CHEBI_23414
    ing8_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_002016 --> ing8_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing8_L_Glutamic_acid -.-> CHEBI_16015
    ing9_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_002016 --> ing9_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing9_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing10_Pyridoxine["Pyridoxine"]
    CultureMech_002016 --> ing10_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing10_Pyridoxine -.-> CHEBI_16709
    ing11_Biotin["Biotin"]
    CultureMech_002016 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Distilled_water["Distilled water"]
    CultureMech_002016 --> ing12_Distilled_water
    ing13_Glycerol["Glycerol"]
    CultureMech_002016 --> ing13_Glycerol

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Ammonium Sulfate
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
2 Monopotassium Phosphate
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
3 Disodium Phosphate
HO4P.2Na 141.958 g/mol
2.5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
4 Sodium citrate
C6H5O7.3Na 258.069 g/mol
0.1 G_PER_L CHEBI:53258
sodium citrate
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
5 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.05 G_PER_L CHEBI:32599
magnesium sulfate
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
6 Calcium Chloride
Ca.2Cl 110.984 g/mol
0.0005 G_PER_L CHEBI:3312
calcium dichloride
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
7 Zinc Sulfate
O4S.Zn 161.453 g/mol
0.001 G_PER_L CHEBI:35176
zinc sulfate
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
8 Copper Sulfate
Cu.O4S 159.609 g/mol
0.001 G_PER_L CHEBI:23414
copper(II) sulfate
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
9 L-Glutamic acid
C5H9NO4 147.13 g/mol
0.5 G_PER_L CHEBI:16015
L-glutamic acid
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
10 Ferric ammonium citrate 0.04 G_PER_L CHEBI:31604
ferric ammonium citrate
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
11 Pyridoxine
C8H11NO3 169.18 g/mol
0.001 G_PER_L CHEBI:16709
pyridoxine
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
12 Biotin
C10H16N2O3S 244.316 g/mol
0.0005 G_PER_L CHEBI:15956
biotin
 Basal constituent rate for the source's explicitly one-liter final medium; commercial 4.7 g powder represents this same base, not an additional ingredient.
13 Distilled water 0.9 L Source preparation input, 0.9 L; water is 900 ml and glycerol is 2 ml. The API table's 1000 ml water row conflicts with its preparation prose and the original PDF; this input follows the explicit preparation, not a sum of component volumes.
14 Glycerol 0.002 L Source preparation input, 0.002 L; water is 900 ml and glycerol is 2 ml. The API table's 1000 ml water row conflicts with its preparation prose and the original PDF; this input follows the explicit preparation, not a sum of component volumes.

Preparation

  1. Solution g/liter (final solution) 4.7 g/l. Final pH at 25°C 6.6 ± 0.2.
  2. To prepare one liter of final medium, suspend the amount of dehydrated powder indicated under FORMULAE-Solution (and on the package label) in 900 ml distilled and deionized water containing 2 ml glycerol and heat to boiling to dissolve completely. Sterilize in the autoclave for 10 or 15 min at 15 Ibs pressure (121°C), per label copy. Cool to 50-55°C.
  3. Aseptically add 100 ml of the appropriate ADC Enrichment and mix throughly.

Solutions

ADC Enrichment

Dose: 0.1 L

Stock composition
IngredientConcentrationNotes
Bovine albumin 50 G_PER_L ADC stock preparation: MediaDive explicitly reports 50 g/L for this stock from 5 g in its 100 ml stock encoding; not a finished-medium rate. Source material qualifier: albumin fraction V.
Dextrose 20 G_PER_L ADC stock preparation: MediaDive explicitly reports 20 g/L for this stock from 2 g in its 100 ml stock encoding; not a finished-medium rate.
Catalase 0.003 G_PER_L ADC stock preparation: MediaDive explicitly reports 0.003 g/L for this stock from 0.3 mg in its 100 ml stock encoding; not a finished-medium rate. Source material qualifier: Beef.
Distilled water 0.1 L ADC stock preparation: 100 ml water = 0.1 L input, not an asserted final-volume marker.
100 ml = 0.1 L enrichment addition after cooling; its stock composition below is not finished-medium concentration.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable).
2026-01-26T17:53:12.528434Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 857
2026-03-13T06:19:09.433755Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002016
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.994750+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BACTO MIDDLEBROOK 7H9 BROTH" to "bacto_middlebrook_7h9_broth" for consistent matching
2026-05-20T06:01:09.567977+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.4-6.8' -> {'min': 6.4, 'max': 6.8}
2026-06-05T07:40:50.465105+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-09T00:43:54.768901+00:00 codex RESTORED_MIDDLEBROOK_INGREDIENT_AND_STOCK_SCOPES_WITH_EVIDENCE Restore albumin and separate basal inputs, commercial-base detail and ADC/OADC stock amounts/doses. Correct volume/mass confusion; retain unsupported mass/percent bases and source-version conflicts explicitly. Preserve IDs and all prior history.