methanothermobacter_methanobacterium_transduction_medium

Overview

External ontology

mediadive.medium:863 (source link unavailable) — METHANOTHERMOBACTER/METHANOBACTERIUM TRANSDUCTION MEDIUM

Applications

• Microbial cultivation

pH

7.0

High-metal flag

True

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium863.pdf

Composition

flowchart LR
    CultureMech_002022["methanothermobacter_methanobacterium_transduction_medium"]
    ing0_Agar["Agar"]
    CultureMech_002022 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002022 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002022 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Resazurin["Resazurin"]
    CultureMech_002022 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002022 --> ing4_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002022 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002022 --> ing6_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing7_Na2WO4_x_6_H2O["Na2WO4 x 6 H2O"]
    CultureMech_002022 --> ing7_Na2WO4_x_6_H2O
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002022 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2Se2O3["Na2Se2O3"]
    CultureMech_002022 --> ing9_Na2Se2O3
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002022 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002022 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_Na2CO3["Na2CO3"]
    CultureMech_002022 --> ing12_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing12_Na2CO3 -.-> CHEBI_29377
    ing13_L_Cysteine_HCl["L-Cysteine HCl"]
    CultureMech_002022 --> ing13_L_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing13_L_Cysteine_HCl -.-> CHEBI_91247
    ing14_Titanium_III__chloride["Titanium(III) chloride"]
    CultureMech_002022 --> ing14_Titanium_III__chloride
    ing15_Sodium_citrate["Sodium citrate"]
    CultureMech_002022 --> ing15_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing15_Sodium_citrate -.-> CHEBI_53258
    ing16_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002022 --> ing16_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing16_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (17)

Preparation

1. Anaerobically dispense liquid minimal medium in 10-ml portions into 100-ml serum vials under an atmosphere of N2/CO2 (80:20). Seal vials with butyl rubber stoppers secured with aluminium crimp seals. Sterilize by autoclaving for 15 minutes at 121°C. Prior to inoculation, pressurize bottles to 2 x 102 kPa with H2/CO2. For solidified plating medium, add the following components per liter of liquid minimal medium:
2. Add titanium(III) citrate solution to warm (60°C) agar medium after autoclaving. For preparation of solidified agar medium.
3. Adjust pH of nitrilotriacetic acid in solution to 7.0 with KOH. Add minerals and adjust the final pH to 7.0.
4. Dissolve 106 g Na2CO3 in 1 liter of degassed H2O. Autoclave in sealed serum bottles under CO2.
5. To 50 ml of boiled and cooled distilled or deionized H2O add 5 g of L-cysteine hydrochloride . Adjust pH to 10 with 3N NaOH and store under N2 in a serum bottle sealed with a butyl rubber stopper.
6. Neutralize pH with a saturated sodium carbonate solution and store in a serum bottle under N2.
7. To 50 ml of boiled and cooled distilled or deionized H2O add 5 g of Na2S x 9 H2O. Adjust pH to 10 with 3N NaOH and store under N2 in a serum bottle sealed with a butyl rubber stopper.

Solutions

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• methanothermobacter_methanobacterium_transduction_medium (CultureMech:002022)