methanothermobacter_methanobacterium_transduction_medium

CultureMech:002022 archaea DEFINED SOLID_AGAR
Original name: METHANOTHERMOBACTER/METHANOBACTERIUM TRANSDUCTION MEDIUM

Overview

External ontology
mediadive.medium:863 (source link unavailable) — METHANOTHERMOBACTER/METHANOBACTERIUM TRANSDUCTION MEDIUM
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium863.pdf

Composition

flowchart LR
    CultureMech_002022["methanothermobacter_methanobacterium_transduction_medium"]
    ing0_Agar["Agar"]
    CultureMech_002022 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002022 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002022 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Resazurin["Resazurin"]
    CultureMech_002022 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002022 --> ing4_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002022 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002022 --> ing6_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing7_Na2WO4_x_6_H2O["Na2WO4 x 6 H2O"]
    CultureMech_002022 --> ing7_Na2WO4_x_6_H2O
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002022 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2Se2O3["Na2Se2O3"]
    CultureMech_002022 --> ing9_Na2Se2O3
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002022 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002022 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_Na2CO3["Na2CO3"]
    CultureMech_002022 --> ing12_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing12_Na2CO3 -.-> CHEBI_29377
    ing13_L_Cysteine_HCl["L-Cysteine HCl"]
    CultureMech_002022 --> ing13_L_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing13_L_Cysteine_HCl -.-> CHEBI_91247
    ing14_Titanium_III__chloride["Titanium(III) chloride"]
    CultureMech_002022 --> ing14_Titanium_III__chloride
    ing15_Sodium_citrate["Sodium citrate"]
    CultureMech_002022 --> ing15_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing15_Sodium_citrate -.-> CHEBI_53258
    ing16_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002022 --> ing16_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing16_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Agar 14.7638 G_PER_L CHEBI:2509
agar
2 KH2PO4
H2O4P.K 136.084 g/mol
68 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
21.2 G_PER_L CHEBI:31206
ammonium chloride
4 Resazurin
C12H7NO4 229.191 g/mol
0.01 G_PER_L CHEBI:8806
Resazurin
5 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
9.55 G_PER_L CHEBI:44557
nitrilotriacetic acid
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.06 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
0.98 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
8 Na2WO4 x 6 H2O 0.264 G_PER_L
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.118 G_PER_L CHEBI:34887
nickel dichloride
10 Na2Se2O3 0.1 G_PER_L
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.023 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 Na2CO3
CO3.2Na 105.988 g/mol
106 G_PER_L CHEBI:29377
sodium carbonate
14 L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
100 G_PER_L CHEBI:91247
L-cysteine hydrochloride
15 Titanium(III) chloride 0.75 G_PER_L
16 Sodium citrate
C6H5O7.3Na 258.069 g/mol
2.5807 G_PER_L CHEBI:53258
sodium citrate
17 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
100 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Anaerobically dispense liquid minimal medium in 10-ml portions into 100-ml serum vials under an atmosphere of N2/CO2 (80:20). Seal vials with butyl rubber stoppers secured with aluminium crimp seals. Sterilize by autoclaving for 15 minutes at 121°C. Prior to inoculation, pressurize bottles to 2 x 102 kPa with H2/CO2. For solidified plating medium, add the following components per liter of liquid minimal medium:
  2. Add titanium(III) citrate solution to warm (60°C) agar medium after autoclaving. For preparation of solidified agar medium.
  3. Adjust pH of nitrilotriacetic acid in solution to 7.0 with KOH. Add minerals and adjust the final pH to 7.0.
  4. Dissolve 106 g Na2CO3 in 1 liter of degassed H2O. Autoclave in sealed serum bottles under CO2.
  5. To 50 ml of boiled and cooled distilled or deionized H2O add 5 g of L-cysteine hydrochloride . Adjust pH to 10 with 3N NaOH and store under N2 in a serum bottle sealed with a butyl rubber stopper.
  6. Neutralize pH with a saturated sodium carbonate solution and store in a serum bottle under N2.
  7. To 50 ml of boiled and cooled distilled or deionized H2O add 5 g of Na2S x 9 H2O. Adjust pH to 10 with 3N NaOH and store under N2 in a serum bottle sealed with a butyl rubber stopper.

Solutions

Sodium carbonate solution

Dose: 59.7638 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.550665Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 863
2026-03-09T08:04:22.046078+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:09.495934Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002022
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.064225+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOTHERMOBACTER/METHANOBACTERIUM TRANSDUCTION MEDIUM" to "methanothermobacter_methanobacterium_transduction_medium" for consistent matching
2026-05-16T09:04:22.845977+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:32.848134+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.460011+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.