chloroflexus_medium_modified

Overview

External ontology

mediadive.medium:87 (source link unavailable) — CHLOROFLEXUS MEDIUM (modified)

Applications

• Microbial cultivation

pH

8.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87.pdf

Composition

flowchart LR
    CultureMech_002038["chloroflexus_medium_modified"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002038 --> ing0_Yeast_extract
    ing1_Glycyl_glycine["Glycyl-glycine"]
    CultureMech_002038 --> ing1_Glycyl_glycine
    CHEBI_17201["CHEBI:17201"]
    ing1_Glycyl_glycine -.-> CHEBI_17201
    ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_002038 --> ing2_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002038 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KNO3["KNO3"]
    CultureMech_002038 --> ing4_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing4_KNO3 -.-> CHEBI_63043
    ing5_NaNO3["NaNO3"]
    CultureMech_002038 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_NaCl["NaCl"]
    CultureMech_002038 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002038 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_002038 --> ing8_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing8_Fe_III__citrate -.-> CHEBI_144421
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002038 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002038 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002038 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002038 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002038 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002038 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002038 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002038 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

Preparation

1. Adjust pH to 8.2.
2. Boil the medium under a stream of nitrogen gas for a few minutes and distribute 10 ml medium into 15 ml hungate tubes. Bubble each tube with nitrogen gas and close immediately with a rubber septum and screw tight.
3. Autoclave at 121 °C for 15 min.
4. After autoclaving inject 0.1 ml of neutralized sulfide solution (0.03% end concentration) to each tube.
5. The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• chloroflexus_medium_modified (CultureMech:002038)