chloroflexus_medium_modified

CultureMech:002038 bacterial COMPLEX LIQUID
Original name: CHLOROFLEXUS MEDIUM (modified)

Overview

External ontology
mediadive.medium:87 (source link unavailable) — CHLOROFLEXUS MEDIUM (modified)
Applications
  • Microbial cultivation
pH
8.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87.pdf

Composition

flowchart LR
    CultureMech_002038["chloroflexus_medium_modified"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002038 --> ing0_Yeast_extract
    ing1_Glycyl_glycine["Glycyl-glycine"]
    CultureMech_002038 --> ing1_Glycyl_glycine
    CHEBI_17201["CHEBI:17201"]
    ing1_Glycyl_glycine -.-> CHEBI_17201
    ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_002038 --> ing2_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002038 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KNO3["KNO3"]
    CultureMech_002038 --> ing4_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing4_KNO3 -.-> CHEBI_63043
    ing5_NaNO3["NaNO3"]
    CultureMech_002038 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_NaCl["NaCl"]
    CultureMech_002038 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002038 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_002038 --> ing8_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing8_Fe_III__citrate -.-> CHEBI_144421
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002038 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002038 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002038 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002038 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002038 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002038 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002038 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002038 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.94697 G_PER_L
2 Glycyl-glycine
C4H8N2O3 132.119 g/mol
0.94697 G_PER_L CHEBI:17201
glycylglycine
3 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
0.094697 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.094697 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 KNO3
K.NO3 101.102 g/mol
0.094697 G_PER_L CHEBI:63043
potassium nitrate
6 NaNO3
NO3.Na 84.994 g/mol
0.473485 G_PER_L CHEBI:63005
sodium nitrate
7 NaCl
Cl.Na 58.443 g/mol
0.094697 G_PER_L CHEBI:26710
sodium chloride
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0473485 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 Fe(III) citrate
C6H5FeO7 244.944 g/mol
5 G_PER_L CHEBI:144421
iron(III) citrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
30 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 8.2.
  2. Boil the medium under a stream of nitrogen gas for a few minutes and distribute 10 ml medium into 15 ml hungate tubes. Bubble each tube with nitrogen gas and close immediately with a rubber septum and screw tight.
  3. Autoclave at 121 °C for 15 min.
  4. After autoclaving inject 0.1 ml of neutralized sulfide solution (0.03% end concentration) to each tube.
  5. The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.137211Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 87
2026-03-13T06:19:09.669627Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002038
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.255190+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CHLOROFLEXUS MEDIUM (modified)" to "chloroflexus_medium_modified" for consistent matching
2026-06-05T07:40:53.336580+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.630235+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:54.485452+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.