Overview
- External ontology
- mediadive.medium:942 (source link unavailable) — AGAR WITH E. COLI AS FEED
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium942.pdf
Composition
flowchart LR
CultureMech_002118["agar_with_e_coli_as_feed"]
ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002118 --> ing0_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
ing1_Agar["Agar"]
CultureMech_002118 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 2 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.2, autoclave and pour plates. Grow Escherichia coli (e.g. DSM 498) in liquid culture, collect cells by centrifugation and resuspend them in enough water to receive a thick but ready-to-pipet suspension. Autoclave. Place three separate drops on one CaCl2-agar plate and streak every drop once to receive E. coli lines, about 3 cm long, across the E. coli drops. Inoculate E. coli lines/drops with myxobacteria.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- agar_with_e_coli_as_feed (CultureMech:002118)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.839313Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 942 |
| 2026-03-13T06:19:10.472501Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002118 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.143439+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AGAR WITH E. COLI AS FEED" to "agar_with_e_coli_as_feed" for consistent matching |
| 2026-06-05T07:40:47.597470+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:22.170929+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 00f65b019aa693dbea23c7b7fe2141f84b3518ea9d455e15aa62e89af3742e1d |